Simultaneous determination of tetrahydrocortisol and tetrahydrocortisone in human plasma and urine by stable isotope dilution mass spectrometry

Journal of Chromatography. B, Biomedical Sciences and Applications
T FurutaY Kasuya

Abstract

A capillary gas chromatographic-mass spectrometric method for the simultaneous determination of tetrahydrocortisol (THF, 3alpha,11beta,17alpha,21-tetrahydroxy-5beta-preg nane-20-one), allo-tetrahydrocortisol (allo-THF, 3alpha,11beta,17alpha,21-tetrahydroxy-5alpha-pre gnane-20-one) and tetrahydrocortisone (THE, 3alpha,17alpha,21-trihydroxy-5beta-pregnane-11,20-dion e) in human plasma and urine is described. [1,2,3,4,5-2H5]THF (THF-d5), allo-[1,2,3,4,5-2H5]THF (allo-THF-d5) and [1,2,3,4,5-2H5]THE (THE-d5) were used as internal standards. A double derivatization (bismethylenedioxypentafluoropropionate, BMD-PFP) made possible the separation of the three tetrahydrocorticoids with good gas chromatographic behavior. Quantitation was carried out by selected-ion monitoring of the characteristic fragment ions ([M-30]+) of the BMD-PFP derivatives of THF, allo-THF and THE. The sensitivity, specificity, precision and accuracy of the method were demonstrated to be satisfactory for measuring low concentrations of THF, allo-THF and THE in human plasma and urine.

References

Jan 1, 1990·The Journal of Clinical Endocrinology and Metabolism·S UlickF Mantero
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Citations

Sep 5, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Atsushi SuzukiTakashi Furuta
Sep 10, 2011·The Journal of Endocrinology·Mark NixonRuth Andrew
Apr 1, 2014·Chromatographia·Fidel AllendeCarlos E Fardella
Jan 8, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Radmila PavlovicLuca Chiesa
May 1, 2009·Obesity Research & Clinical Practice·Nozomi KobayashiKazuwa Nakao
Aug 15, 2009·Rapid Communications in Mass Spectrometry : RCM·Angela CuzzolaPiero Salvadori
Jan 5, 2002·Journal of Chromatography. a·K ShimadaT Higashi

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