PMID: 11312879Apr 21, 2001Paper

Simultaneous determination of thiamin and riboflavin in mushrooms by liquid chromatography

Journal of Agricultural and Food Chemistry
M J EsteveJ M García-Cantabella

Abstract

A simple, fast, inexpensive, and reliable method useful for the simultaneous, routine determination of thiamin and riboflavin in mushrooms is examined. It uses the extraction procedure, with slight modifications, proposed by the AOAC for the extraction of thiamin and riboflavin, followed by a liquid chromatographic separation on a reversed-phase Spherisorb ODS column with methanol/water as mobile phase gradient. Fluorometric detection is used at the following excitation and emission wavelengths, respectively, 360 and 425 nm in the case of thiamin and 422 and 515 nm for riboflavin. The analytical parameters of linearity, the precision of the method (RSD = 2.45 and 2.51% for thiamin and riboflavin, respectively), and the results of the comparison with the corresponding AOAC fluorometric methods show that the studied method is useful for the measurement of thiamin and riboflavin in fresh mushrooms.

References

May 1, 1980·Journal of Agricultural and Food Chemistry·G Piombo, Y F Lozano

❮ Previous
Next ❯

Citations

Feb 22, 2012·Analytical and Bioanalytical Chemistry·Pilar ViñasManuel Hernández-Córdoba
Apr 23, 2009·Prikladnaia biokhimiia i mikrobiologiia·T A MisharinaN I Krikunova
Mar 16, 2012·Journal of Food Science·Alicia del Carmen Mondragón-PortocarreroMa Ángeles Romero-Rodríguez
Mar 12, 2019·International Journal of Food Science·Mohammad F HossainSusan L Mayhew

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.