Simultaneous determination of total plasma glutathione, homocysteine, cysteinylglycine, and methionine by high-performance liquid chromatography with electrochemical detection

Journal of Clinical Laboratory Analysis
P HouzeB Gourmel

Abstract

We here describe an ion-exchange high-performance liquid chromatography technique with electrochemical detection for rapid quantification of glutathione, homocysteine, cysteinylglycine, and methionine. The analytical validation of the technique showed within-assay and between-assay coefficients of variation between 3.1 and 4.3%, and 3.7 and 8.6%, respectively. Percentages of recovery for overload and dilution tests were between 87 and 120%. Detection limits were 1 micromol/L for methionine and 0.5 micromol/L for other compounds. There was no interference with any physiological and pharmacological substances possessing a thiol function. Aminothiol concentrations determined in 100 control subjects (50 women and 50 men) showed no age- or sex-rated differences for except for homocysteine which was increased (+ 28%) in oldest subjects of both sexes. In 60 patients at risk (30 with chronic renal failure, 30 with diabetes), homocysteine concentration was significantly increased. No variation in other aminothiols was observed in diabetic subjects. Methionine was decreased and cysteinylglycine was increased in patients with chronic renal failure. The present technique-rapid, easy to use, and reliable-appears suitable for routine applica...Continue Reading

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Citations

Jun 22, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·C MislanovaM Obolenskaya
Apr 20, 2006·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Elin JerremalmHans Ehrsson
Jan 26, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jérôme BouligandGilles Vassal
Jun 6, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Haibing Li, Xiaoqiong Wang
Jul 15, 2015·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Patricia T Lee, Richard G Compton
Nov 3, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Wei-Lung TsengHuan-Tsung Chang

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