PMID: 11339272May 8, 2001Paper

Simultaneous determination of Ziagen and its phosphorylated metabolites by ion-pairing high-performance liquid chromatography-tandem mass spectrometry

Journal of Chromatography. B, Biomedical Sciences and Applications
E N FungAchintya K Sinhababu

Abstract

An ion-paring HPLC-MS-MS method with positive ion mode electrospray ionization has been developed to simultaneously quantify Ziagen, carbovir monophosphate, carbovir diphosphate and carbovir triphosphate. N',N'-Dimethylhexylamine was used as the ion-pairing agent. The presence of this ion-pairing agent allowed the retention and separation of the four compounds on a reversed-phase HPLC column as well as the detection of the nucleotides with positive ion mode electrospray ionization. The limits of detection were found to be better than 25 nM for all the analytes. Calibration curves of the analytes showed excellent linearity over the range of 25 nM to 5 microM. The relative standard deviations and accuracies for replicate analyses of quality control samples were less than 15%. The method has been successfully applied to the analysis of these compounds in human liver cells treated with Ziagen.

Citations

Mar 18, 2009·Pharmaceutical Research·Ping ChenKenneth K Chan
May 14, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gaëlle HenneréHenri Benech
Jul 28, 2006·Antimicrobial Agents and Chemotherapy·Henri BenechJacques J Grassi
Nov 25, 2004·Antimicrobial Agents and Chemotherapy·Brenda I Hernandez-SantiagoRaymond F Schinazi
Jun 28, 2012·Biochemical and Biophysical Research Communications·Xiaoguang LiEiichi N Kodama
Jul 14, 2010·Mass Spectrometry Reviews·Robert S JansenJos H Beijnen
Jul 26, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ang LiuJian Wang
Jul 27, 2001·Journal of Mass Spectrometry : JMS
Apr 7, 2009·Journal of Separation Science·Vincenzo PucciFabio Bonelli
Oct 24, 2003·The Journal of the Association of Nurses in AIDS Care : JANAC·Richard S Ferri, Carl A Kirton
Jun 18, 2021·Critical Reviews in Analytical Chemistry·Sara I AborasMohamed A Korany
Oct 9, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Raquel LorenzettiGilberto de Nucci
Apr 3, 2019·Analytical Chemistry·Xiaorong FuShawn C Burgess

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.