Simultaneous E-cadherin and PLEKHA7 expression negatively affects E-cadherin/EGFR mediated ovarian cancer cell growth

Journal of Experimental & Clinical Cancer Research : CR
Katia ReaAntonella Tomassetti

Abstract

The disruption of E-cadherin-mediated adhesion is considered an important driver of tumor progression. Nevertheless, numerous studies have demonstrated that E-cadherin promotes growth- or invasion-related signaling, contrary to the prevailing notion. During tumor progression, epithelial ovarian cancer (EOC) maintains E-cadherin expression and can positively affect EOC cell growth by contributing to PI3K/AKT activation. In polarized epithelia PLEKHA7, a regulator of the zonula adherens integrity, impinges E-cadherin functionality, but its role in EOCs has been never studied. Ex-vivo EOC cells and cell lines were used to study E-cadherin contribution to growth and EGFR activation. The expression of the proteins involved was assessed by real time RT-PCR, immunohistochemistry and western blotting. Cells growth and drug susceptibility was monitored in different 3-dimensional (3D) systems. Recombinant lentivirus-mediated gene expression, western blotting, immunoprecipitation and confocal microscopy were applied to investigate the biological impact of PLEKHA7 on E-cadherin behaviour. The clinical impact of PLEKHA7 was determined in publicly available datasets. We show that E-cadherin expression contributes to growth of EOC cells and f...Continue Reading

References

Feb 20, 1993·International Journal of Cancer. Journal International Du Cancer·C A van den Berg-BakkerP I Schrier
Aug 14, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Richard W TothillDavid D L Bowtell
Dec 3, 2008·Neoplasia : an International Journal for Oncology Research·Antonella TomassettiSilvana Canevari
Jul 24, 2010·The American Journal of Pathology·Ernst Lengyel
Dec 7, 2010·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Nelly Auersperg
Nov 22, 2011·Nature Medicine·Bogdan MateescuFatima Mechta-Grigoriou
May 24, 2012·The EMBO Journal·Tomas ValentaKonrad Basler
May 16, 2014·Nature Reviews. Molecular Cell Biology·Masatoshi Takeichi
May 16, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·L De CeccoL Licitra
Jan 24, 2015·Organic & Biomolecular Chemistry·Fabio DoroMonica Civera
Jun 9, 2015·Gynecologic Oncology·Anirban K MitraJoanna E Burdette
Oct 7, 2015·Trends in Cell Biology·Xin Ye, Robert A Weinberg
Aug 27, 2016·International Journal of Molecular Sciences·Francesca RoggianiAntonella Tomassetti
Jun 12, 2017·Neoplasia : an International Journal for Oncology Research·Yuliya KlymenkoM Sharon Stack

❮ Previous
Next ❯

Citations

Jun 7, 2020·Clinical Epigenetics·Nathan A DamaschkeDavid F Jarrard
Apr 11, 2020·International Journal of Molecular Sciences·Joyce Nair-MenonAntonis Kourtidis
Dec 20, 2020·Life Sciences·Mahmood BaraniGeorge Z Kyzas
Jun 20, 2020·Biochimica Et Biophysica Acta. Biomembranes·Florian RouaudSandra Citi
Aug 7, 2021·International Journal of General Medicine·Fengjuan ShiJianxun Lu
Sep 9, 2021·Bioscience Reports·Jiahui WeiJiabao Zhang

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE19352
GSE26193

Methods Mentioned

BETA
FCS
biopsies
xenografts
Immunoprecipitation
Assay
transfection
flow cytometry

Software Mentioned

ImageJ
GraphPad Prism
CompuSyn
Leica LAS AF
Adobe Photoshop

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.