Simultaneous Editing of Two Copies of Gh14-3-3d Confers Enhanced Transgene-Clean Plant Defense Against Verticillium dahliae in Allotetraploid Upland Cotton

Frontiers in Plant Science
Zhennan ZhangJiahe Wu

Abstract

Gossypium hirsutum is an allotetraploid species, meaning that mutants that are difficult to be generated by classical approaches due to gene redundancy. The CRISPR/Cas9 genome editing system is a robust and highly efficient tool for generating target gene mutants, by which the genes of interest may be functionally dissected and applied through genotype-to-phenotype approaches. In this study, the CRISPR/Cas9 genome editing system was developed in G. hirsutum through editing the Gh14-3-3d gene. In T0 transgenic plants, lots of insertions and deletions (indels) in Gh14-3-3d at the expected target site were detected in the allotetraploid cotton At or Dt subgenomes. The results of the PCR, T7EI digestion and sequencing analyses showed that the indels in Gh14-3-3d gene can be stably transmitted to the next generation. Additionally, the indels in the At and Dt subgenomes were segregated in the T1 transgenic plants following Mendelian law, independing on the T-DNA segregation. Two homozygous Gh14-3-3d-edited plants free of T-DNA were chosen by PCR and sequencing assays in the T1 plants, which were called transgene-clean editing plants and were designated ce1 and ce2 in the T2 lines showed higher resistance to Verticillium dahliae infes...Continue Reading

References

Mar 1, 2003·The Plant Journal : for Cell and Molecular Biology·Hideo NakashitaShigeo Yoshida
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J F Wendel
May 2, 2007·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Haiyan ShiXuebao Li
Aug 8, 2007·Developmental Cell·Srinivas S GampalaZhi-Yong Wang
Jan 18, 2011·The Plant Journal : for Cell and Molecular Biology·Sally-Ann WalfordElizabeth S Dennis
Nov 30, 2011·Plant Physiology·Fenglin DengXianlong Zhang
Mar 23, 2012·The Plant Cell·Cécile VrietChristophe Reuzeau
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Aug 28, 2012·Nature Genetics·Kunbo WangShuxun Yu
Jan 5, 2013·Science·Le CongFeng Zhang
May 15, 2013·The Plant Journal : for Cell and Molecular Biology·Ling MinXianlong Zhang
Aug 10, 2013·Nature Biotechnology·Qiwei ShanCaixia Gao
Aug 21, 2013·Cell Research·Zhengyan FengJian-Kang Zhu
Oct 15, 2013·G3 : Genes - Genomes - Genetics·Santosh Kumar UpadhyayRakesh Tuli
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Zhengyan FengJian-Kang Zhu
Mar 1, 2014·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Zhen LiangCaixia Gao
May 20, 2014·Nature Genetics·Fuguang LiShuxun Yu
May 20, 2014·The Plant Journal : for Cell and Molecular Biology·Friedrich FauserHolger Puchta
May 29, 2014·Trends in Plant Science·Xueying GuanZ Jeffrey Chen
Jun 18, 2015·Plant Cell Reports·Shaohui WangXia Cui
Aug 8, 2015·Journal of Cellular Physiology·Chao Li, Baohong Zhang

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Citations

Jul 19, 2019·Journal of Cellular Physiology·Niaz AhmadBaohong Zhang
Aug 14, 2020·International Journal of Molecular Sciences·Sunny AhmarKi-Hong Jung
Aug 2, 2018·Frontiers in Plant Science·Deepa JaganathanGayatri Venkataraman
Aug 23, 2019·International Journal of Molecular Sciences·Ali RazzaqFaiz Ahmad Joyia
Oct 18, 2020·Plants·Bed Prakash Bhatta, Subas Malla
Sep 30, 2020·Trends in Biotechnology·Renhai PengBaohong Zhang
Jan 26, 2020·Trends in Plant Science·Zuoren YangFuguang Li
Apr 2, 2021·BMC Plant Biology·Na SangYuqiang Sun
Apr 4, 2021·International Journal of Molecular Sciences·Sweta NidhiVijay Tripathi
Apr 13, 2021·Journal of Advanced Research·Dangquan ZhangBaohong Zhang
Apr 10, 2021·Journal of Zhejiang University. Science. B·Chao LiBaohong Zhang
Jun 1, 2021·In Vitro Cellular & Developmental Biology. Plant : Journal of the Tissue Culture Association·Yan ZhangGuoquan Liu
Jun 19, 2021·Transgenic Research·Cláudia RatoPaula Rodrigues Oblessuc
Aug 23, 2021·Molecular Biotechnology·Dipinte GuptaRajiv Ranjan

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Methods Mentioned

BETA
dissection
transgenic
PCR
Assay
electrophoresis

Software Mentioned

Cas
Designer

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