Simultaneous electrochemical detection of cervical cancer markers using reduced graphene oxide-tetraethylene pentamine as electrode materials and distinguishable redox probes as labels

Biosensors & Bioelectronics
Dan WuBin Du

Abstract

A novel, highly sensitive electrochemical immunoassay was proposed for the simultaneous determination of carcinoembryonic antigen (CEA) and squamous cell carcinoma antigen (SCCA) for the diagnosis of cervical cancer. Using an electrochemical analysis technique, two well-separated peaks were generated by neutral red and thionine, making the simultaneous detection of the two analytes on the electrode possible. Reduced graphene oxide-tetraethylene pentamine (rGO-TEPA), containing more amino groups, was of benefit to immobilize the primary antibody (Ab1) through an amidation reaction. Au@mesoporous carbon CMK-3 was synthesized and incubated with two secondary antibodies (Ab2) and different redox probes (neutral red and thionine) to fabricate the electrochemical immunosensor label intending to improve the analytical performance of the immunosensor. The immunosensor was prepared with a sandwich structure based on the peak current change of neutral red and thionine before and after the antigen-antibody reaction. The results showed that the immunosensor had a wide linear range, low detection limit, good reproducibility and stability. The method has been applied to the analysis of serum samples with satisfactory results.

References

Jan 12, 2010·Chemical Communications : Chem Comm·Xuemei LiShusheng Zhang
Dec 7, 2011·Chemical Society Reviews·Yuxin LiuPeng Chen

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Citations

Jun 2, 2015·Expert Review of Molecular Diagnostics·Zhanfang Ma, Na Liu
Nov 19, 2015·ACS Nano·Jitendra N TiwariKwang S Kim
Jul 17, 2015·Journal of Biomedical Materials Research. Part a·Xiaoming LiFumio Watari
Apr 8, 2017·Sensors·Benoît Piro, Steeve Reisberg
May 6, 2017·Sensors·Mingfei PanShuo Wang
Apr 8, 2016·Chemical Society Reviews·Adriano AmbrosiMartin Pumera
Apr 14, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Liyuan WangZhanfang Ma
Apr 9, 2020·Sensors·Mateusz Kondzior, Iwona Grabowska
Oct 31, 2020·International Journal of Biological Macromolecules·Zahra KarimzadehBalal Khalilzadeh
Jun 18, 2021·Advanced Materials·Xinyi LiuWanwan Li

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