Simultaneous electrokinetic and hydrodynamic injection and sequential stacking featuring sweeping for signal amplification following MEKC during the analysis of rapamycin (sirolimus) in serum samples

Electrophoresis
Ilona OlędzkaTomasz Baczek

Abstract

Simultaneous electrokinetic and hydrodynamic injection of rapamycin (sirolimus) with off-line and online sample preconcentration techniques and using MEKC has been studied. Compared to conventional hydrodynamic injection, a 168-fold improvement in the signal was obtained with a combination of simultaneous electrokinetic and hydrodynamic injectionand field enhanced sample injection in conjunction with a sweeping technique called sequential stacking featuring sweeping. However, the coupling of the developed electrophoretic method and solid-phase microextraction allowed the signal intensity to increase more than 231 times. In this approach, the injection of the sample at negative polarity (anode at the detector end) into the capillary and the MEKC separation was achieved within 5 min using an electrolyte (composed of 10 mM sodium tetraborate and 40 mM SDS) when ultraviolet (UV) detection was performed at 280 nm. Thus, by combining the application of the sequential stacking featuring sweeping supported by the solid-phase microextraction clean-up procedure, the detection limit (LOD) for rapamycin in a serum sample was significantly decreased, and was set at 25 ng/mL. The proposed combined simultaneous electrokinetic and hydrodynamic...Continue Reading

References

Sep 16, 1999·Journal of Chromatography. a·F HissnerK Heinig
Sep 19, 2002·Journal of Chromatography. a·Joselito P QuirinoShigeru Terabe
Dec 18, 2002·Journal of Pharmaceutical and Biomedical Analysis·Jong-Bok Kim, Shigeru Terabe
Jul 28, 2004·Analytical and Bioanalytical Chemistry·Wolfgang BuchbergerThuy Diep Thanh Vo
Sep 22, 2005·Therapeutic Drug Monitoring·Vincent H Mabasa, Mary H H Ensom
Feb 27, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·GholamAli KhoschsorurGabriele Halwachs-Baumann
Aug 23, 2011·Journal of Chromatography. a·Michelle M MeighanMichael C Breadmore
Nov 18, 2011·Journal of Pharmaceutical and Biomedical Analysis·Alan R OylerYuri V Il'ichev
Sep 22, 2012·Journal of Chromatography. a·Heide R RabanesJoselito P Quirino
Apr 27, 2013·Journal of Chromatographic Science·Dongli DengYingying Su
Jun 19, 2013·Journal of Chromatography. a·Joselito P Quirino
May 6, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Anne SeidlitzWerner Weitschies
Jul 21, 2015·Journal of Chromatography. a·Daniel GstoettenmayrMichael Breadmore
Feb 15, 2016·Journal of Pharmaceutical and Biomedical Analysis·Adriana Mika, Piotr Stepnowski

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