Simultaneous Electrophysiology and Fiber Photometry in Freely Behaving Mice.

Frontiers in Neuroscience
Amisha A PatelShuzo Sakata

Abstract

In vivo electrophysiology is the gold standard technique used to investigate sub-second neural dynamics in freely behaving animals. However, monitoring cell-type-specific population activity is not a trivial task. Over the last decade, fiber photometry based on genetically encoded calcium indicators (GECIs) has been widely adopted as a versatile tool to monitor cell-type-specific population activity in vivo. However, this approach suffers from low temporal resolution. Here, we combine these two approaches to monitor both sub-second field potentials and cell-type-specific population activity in freely behaving mice. By developing an economical custom-made system and constructing a hybrid implant of an electrode and a fiber optic cannula, we simultaneously monitor artifact-free mesopontine field potentials and calcium transients in cholinergic neurons across the sleep-wake cycle. We find that mesopontine cholinergic activity co-occurs with sub-second pontine waves, called P-waves, during rapid eye movement sleep. Given the simplicity of our approach, simultaneous electrophysiological recording and cell-type-specific imaging provides a novel and valuable tool for interrogating state-dependent neural circuit dynamics in vivo.

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Citations

Jan 15, 2021·Pharmacology, Biochemistry, and Behavior·Yuanmo WangJacqueline D Keighron
Jan 15, 2021·Biomedical Physics & Engineering Express·Juan Pablo MarcoletaTheodor Doll
Jun 29, 2021·Journal of Micromechanics and Microengineering : Structures, Devices, and Systems·Zeinab RamezaniHui Fang

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Software Mentioned

MATLAB
LabVIEW
MathWorks
Intan

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