Simultaneous enantioseparation and sensitive detection of eight beta-blockers using capillary electrochromatography-electrospray ionization-mass spectrometry

Electrophoresis
Jack Zheng, Shahab A Shamsi

Abstract

The feasibility of using vancomycin chiral stationary phase (CSP) and polar organic eluent is investigated for simultaneous enantioseparation of eight beta-blockers using CEC coupled to ESI mass spectrometric detection (ESI-MS). The internally tapered capillaries were utilized to pack CEC-MS columns. As compared to externally tapered columns, the use of internally tapered columns demonstrated enhanced stability, durability, and reproducibility. A mixture containing methanol/ACN/acetic acid/triethylamine at 70:30:1.6:0.2 v/v/v/v was considered as optimum mobile phase since it provided a good compromise between resolution and analysis time. As expected, sheath liquid and ESI-MS parameters mainly influenced the detection sensitivity. Interestingly, structural information of beta-blockers was available by varying the MS fragmentor voltage using in-house CID in the scan mode. In order to maximize the chiral/achiral resolution, various column-coupling approaches using teicoplanin as complementary CSP to vancomycin were tested. Several changes in the elution order of beta-blockers were observed using multimodal CSPs with some improvement in chiral or achiral resolution. The quantitative aspects of the CEC-MS method were demonstrated u...Continue Reading

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Citations

May 4, 2010·Analytical and Bioanalytical Chemistry·Leena Suntornsuk
Jun 17, 2010·Se pu = Chinese journal of chromatography·Zian LinGuonan Chen
Nov 1, 2011·Journal of Chromatography. a·William Bragg, Shahab A Shamsi
Aug 19, 2008·Journal of Chromatography. a·Gerald Gübitz, Martin G Schmid
Apr 11, 2009·Electrophoresis·Alessandro GiuffridaAlejandro Cifuentes
Aug 5, 2009·Electrophoresis·Peter Mikus, Katarína Maráková
Jul 16, 2008·Journal of Separation Science·Giovanni D'Orazio, Salvatore Fanali
Feb 28, 2007·Journal of Mass Spectrometry : JMS
Jul 4, 2007·Electrophoresis·Beatrix Preinerstorfer, Michael Lämmerhofer
Nov 28, 2007·Electrophoresis·Eun Joo SongYoung Sook Yoo
Dec 29, 2007·Electrophoresis·Andras GasparPhilippe Schmitt-Kopplin
Dec 29, 2007·Electrophoresis·Laura Sánchez-HernándezCarmen García-Ruiz
Dec 29, 2007·Electrophoresis·Yuli Huo, Wim Th Kok
Sep 13, 2011·Electrophoresis·Debby Mangelings, Yvan Vander Heyden
Jun 19, 2013·Mass Spectrometry Reviews·Hanan Awad, Anas El-Aneed
Feb 11, 2014·Journal of Separation Science·Won Jo Cheong
Apr 9, 2010·Electrophoresis·Carolina SimóAlejandro Cifuentes
Nov 16, 2007·Analytical Chemistry·Dean Norton, Shahab A Shamsi

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