Simultaneous estimation of phenolic acids in sea buckthorn (Hippophaë rhamnoides) using RP-HPLC with DAD

Journal of Pharmaceutical and Biomedical Analysis
Ranjith ArimboorC Arumughan

Abstract

A RP-HPLC-DAD method was developed and validated for the simultaneous analysis of nine phenolic acids including gallic acid, protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, salicylic acid, p-coumaric acid, cinnamic acid, caffiec acid and ferulic acid in sea buckthorn (SB) (Hippophaë rhamnoides) berries and leaves. The method was validated in terms of linearity, LOD, precision, accuracy and recovery and found to be satisfactory. Phenolic acid derivatives in anatomical parts of SB berries and leaves were separated into free phenolic acids, phenolic acids bound as esters and phenolic acids bound as glycosides and profiled in HPLC. Berry pulp contained a total of 1068 mg/kg phenolic acids, of which 58.8% was derived from phenolic glycosides. Free phenolic acids and phenolic acid esters constituted 20.0% and 21.2%, respectively, of total phenolic acids in SB berry pulp. The total phenolic acid content in seed kernel (5741 mg/kg) was higher than that in berry pulp and seed coat (Table 2). Phenolic acids liberated from soluble esters constituted the major fraction of phenolic acids (57.3% of total phenolic acids) in seed kernel. 8.4% and 34.3% of total phenolic acids in seed kernel were, respectively contributed by free and...Continue Reading

References

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Citations

Feb 15, 2011·Applied Biochemistry and Biotechnology·Farzaneh RezaeiJean S VanderGheynst
Aug 18, 2010·Analytical and Bioanalytical Chemistry·Saïda El AbdellaouiLudovic Landemarre
Dec 8, 2009·FEBS Letters·C N Vishnu PrasadAnilkumar Gopalakrishnapillai
Mar 26, 2013·Journal of the Science of Food and Agriculture·Lavinia AlexandruGiancarlo Cravotto
Apr 22, 2015·Journal of Agricultural and Food Chemistry·Mirosława TeleszkoTomasz Golis
Feb 12, 2011·Biotechnology Progress·Lawrence D JohJean S Vandergheynst
Mar 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aliona Ghendov-MosanuMarius Niculaua
Jan 24, 2012·International Journal of Food Sciences and Nutrition·Ranjith Arimboor, C Arumughan
Mar 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Adriana CristeNicolae Corcionivoschi
Feb 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mingyue JiMinhui Li
Apr 11, 2009·Journal of Agricultural and Food Chemistry·Yong-Seo ParkShela Gorinstein
Jun 19, 2021·Critical Reviews in Food Science and Nutrition·Yunxiao GaoYilin You
Feb 25, 2020·Journal of Agricultural and Food Chemistry·Zhenyu WangShaoling Lin

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