PMID: 9525258Apr 3, 1998Paper

Simultaneous evaluation of radiation-induced apoptosis and micronuclei in five cell lines

International Journal of Radiation Biology
G Z GuoM Hiraoka

Abstract

This study was conducted to clarify the relationship among the frequencies of micronuclei (MN) and apoptosis, and clonogenic cell survival after irradiation. The frequencies of MN and apoptosis were compared in the surviving fraction in three human tumour cell lines and two rodent cell lines at various irradiation doses. The SHIN-3, DU-145 and CHO-K1 cells showed dose-dependent increases of MN per binucleate cell and an excellent correlation between the MN frequency and surviving fraction after irradiation. The F9 and COLO 320DM cells did not show this correlation. The number of apoptotic cells increased according to the increase in radiation dose in the F9 and COLO 320DM cells, but not in the SHIN-3, DU-145 or CHO-K1 cells. The detection of the MN frequency alone is insufficient to measure cellular intrinsic radiosensitivity. The simultaneous use of the MN assay and the detection of apoptotic cells would be more reliable as a method for predicting cell survival after radiation.

Citations

May 13, 2006·International Journal of Radiation Biology·Ivan PetrovićGiacomo Cuttone
Jul 25, 2006·Oncogene·P ZhangJean Bourhis
Feb 9, 2002·Teratogenesis, Carcinogenesis, and Mutagenesis·Waldemar M PrzybyszewskiOlena Palyvoda
Jan 10, 2004·International Journal of Radiation Biology·M Abend
Apr 9, 2014·International Journal of Radiation Biology·Hiroaki KunogiKeisuke Sasai
Jun 12, 2002·Journal of Radiation Research·Shin-Ichiro Masunaga, Koji Ono
Mar 6, 2003·Journal of Cancer Research and Clinical Oncology·Shin-Ichiro MasunagaHitoshi Hori

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