PMID: 7536324Jan 1, 1995Paper

Simultaneous extraction of total RNA and peptides from tissues: application to tachykinins

Peptides
H P Too, J E Maggio

Abstract

Methods for extraction and isolation of intact RNA are often laborious, time consuming, and preclude the direct analysis of peptides. Similarly, the conditions for extraction and isolation of peptides are unsuitable for the isolation of intact RNA. Thus, to study changes in the levels of neuropeptides and gene expression of the corresponding mRNAs, separate procedures are required. A simple and rapid method for the simultaneous extraction of RNA and peptides from tissues is described. RNA and peptides are extracted with guanidinium isothiocyanate, followed by delipidation, and peptides are isolated by a simple solid-phase extraction procedure. RNA is isolated by differentially partitioning DNA into an organic phase, followed by precipitation with ethanol. The RNA and peptides isolated by this method are of high yield and quality. Furthermore, this method for RNA isolation is successful and efficient, even with tissues that proved recalcitrant with other procedures, and allows the simultaneous processing of multiple samples. We describe the successful application of this procedure for measuring tachykinins and the corresponding preprotachykinin A mRNA from tissues. Extraction of neuropeptide K, a 36-mer tachykinin, was dramatica...Continue Reading

References

Aug 1, 1991·Analytical Biochemistry·I LouveauT D Etherton
Jan 1, 1991·Scandinavian Journal of Gastroenterology. Supplement·H L WaldumB Schulze Søgnen
Jan 1, 1990·Annual Review of Neuroscience·R H Goodman
Apr 27, 1989·Nature·W R Brown
Jan 1, 1988·Annual Review of Neuroscience·J E Maggio
Jun 1, 1971·Saishin igaku. Modern medicine·Y MatsuoT Tsushima
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jan 15, 1982·Biochemical and Biophysical Research Communications·E HannappelB L Horecker

❮ Previous
Next ❯

Citations

Jan 1, 1997·Journal of Clinical Laboratory Analysis·D E Macfarlane, C E Dahle
Nov 1, 1996·Biomedical Chromatography : BMC·T M Phillips
Jun 19, 1998·Neuroreport·D OddiahM Rattray
Sep 2, 2010·Biochemical and Biophysical Research Communications·Guoqiang WanHeng-Phon Too
Dec 20, 2005·Cellular Immunology·Daniel A Nelson, Kenneth L Bost
Jan 6, 1999·Neuroreport·Y W Wong, H P Too

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.