Simultaneous flow cytometric analysis of phagocytosis and oxidative burst activity in equine leukocytes

Veterinary Research Communications
M J B F FlaminioM J Wilkerson

Abstract

This paper describes a method for simultaneously measuring phagocytosis and oxidative burst activity in equine peripheral blood leukocytes by flow cytometry. Opsonized propidium iodide-labelled Staphylococcus aureus (PI-Sa) was used to measure the uptake of bacteria by equine phacocytes and the oxidative burst activity by oxidation of dihydrorhodamine 123. The requirements to achieve optimal activity of phagocytosis and oxidative burst are described. The advantage of the simultaneous technique is that it provides both independent and comparative values for phagocytosis and the oxidative burst, for the detection of impaired mechanisms of microbial destruction. Furthermore, the technique allows evaluation of opsonization activity in this context.

Citations

Sep 1, 2006·Clinical and Vaccine Immunology : CVI·Hyecheong KooYong Ho Park
Jul 15, 2005·Journal of the American Veterinary Medical Association·Alessandra Pellegrini-MasiniM Julia B F Flaminio
Jul 9, 2008·Medical Mycology·John S ReifGregory D Bossart
Aug 29, 2020·PloS One·Mariana Fragoso RentasMarcio Antonio Brunetto
Aug 21, 2003·American Journal of Veterinary Research·Sharon WitonskyS Ansar Ahmed
Oct 1, 2008·Journal of Veterinary Internal Medicine·K A HartM H Barton
Sep 7, 2007·Veterinary Clinical Pathology·Tanya M GrondinKerry S Keeton

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