Simultaneous fluorescent retrograde axonal tracing and immunofluorescent characterization of neurons

Journal of Neuroscience Research
D van der Kooy, H W Steinbusch

Abstract

A simple method is described for simultaneously double labeling single neurons with fluorescent retrograde axonal tracers and immunofluorescence using an antibody to serotonin. Caudata-putamen injection of orange fluorescent propidium iodide, in particular, proved effective in retrogradely labeling dorsal raphe cell bodies. These same perikarya could be stained green with the indirect immunofluorescent method, using green fluorescent fluorescein isothiocynate (FITC) as the second antibody.

References

Apr 1, 1979·Neuroscience Letters·H G KuypersC E Catsman-Berrevoets
Nov 1, 1978·The Journal of Comparative Neurology·M SarP Cuatrecasas
Nov 1, 1977·Neuroscience Letters·H G KuypersR E Padt

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Citations

Jul 1, 1994·Journal of Neuroscience Methods·R Wegerhoff, O Breidbach
May 1, 1983·Neuroscience·M A Ariano, S K Ufkes
Jul 26, 2011·Journal of Chemical Neuroanatomy·José L Lanciego, Floris G Wouterlood
Aug 12, 2014·Journal of Neuroscience Methods·Floris G WouterloodKarl Deisseroth
Feb 18, 2020·Brain Structure & Function·Jose L Lanciego, Floris G Wouterlood
Mar 8, 2021·Neuroscience and Biobehavioral Reviews·Jean-Christophe Cassel, Anne Pereira de Vasconcelos

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