Simultaneous GeneGun immunisation with plasmids encoding antigen and GM-CSF: significant enhancement of murine antivenom IgG1 titres

Vaccine
R A HarrisonR David G Theakston

Abstract

GeneGun DNA immunisation is a potent means of inducing antibody-dominant immune responses that we are exploiting to generate venom toxin-specific antibodies to improve the therapy of systemic envenoming by snakes. Here, we report that mice immunised with DNA encoding the carboxyl domain (JD9) of a haemorrhagic Zn metalloprotease (Jararhagin) in venom of the South American pit viper, Bothrops jararaca, and a plasmid expressing murine cytokine granulocyte/macrophage-colony stimulating factor (GM-CSF) raised significantly higher antigen-specific IgG1 titres than mice immunised with JD9 DNA alone. Serological responses to GeneGun JD9 DNA immunisation were shown to be dominated by IgG1, an IgG subclass associated with T lymphocyte helper 2 (Th2) immune responses. Further significant enhancement of JD9-specific IgG1 titres was achieved by increasing the number of immunisations. This report illustrates that DNA immunisation protocols to achieve high-titre, venom toxin-specific antibody production are well advanced and encourage the development of a DNA-based approach to antivenom production.

References

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Citations

Dec 31, 2003·International Journal of Pharmaceutics·Barnali MandalElke Walter
Nov 7, 2003·Toxicon : Official Journal of the International Society on Toxinology·S S HassonR A Harrison
Jul 24, 2003·Toxicon : Official Journal of the International Society on Toxinology·K BharatiR A Harrison
Mar 27, 2003·Toxicon : Official Journal of the International Society on Toxinology·R A HarrisonR D G Theakston
Jun 2, 2006·PLoS Medicine·Simon C WagstaffRobert A Harrison
Jun 17, 2003·Journal of Toxicology. Clinical Toxicology·David G Lalloo, R David G Theakston
Dec 10, 2002·Analytical Biochemistry·Tianbao ChenChris Shaw
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·Gavin D Laing, Ana M Moura-da-Silva
Nov 8, 2018·Toxins·Erick Bermúdez-MéndezAndreas Hougaard Laustsen
Jul 16, 2008·Toxicon : Official Journal of the International Society on Toxinology·Gabriela Azofeifa-CorderoAlberto Alape-Girón

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