Simultaneous genotyping of alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2) loci by amplified product length polymorphism (APLP) analysis

Electrophoresis
T AoshimaT Suzuki

Abstract

Genotyping of the alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2) loci is important in alcohol studies. We describe a method for simultaneous genotyping of ADH2 and ALDH2 based on amplified product length polymorphism (APLP) analysis. Two polymerase chain reaction (PCR) fragments for ADH2 (57 bp, 53 bp) and two for ALDH2 (78 bp, 73 bp) are simultaneously amplified. Nine banding patterns reflecting the genotypes of the ADH2 and ALDH2 loci are clearly and unambiguously distinguished. The APLP method seems to be particularly suited for large-scale population studies of ADH2 and ALDH2 loci because it is simple and rapid.

References

Jan 1, 1992·Human Genetics·H W GoeddeR Lisker
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Aug 1, 1995·The American Journal of Psychiatry·S HiguchiM Hayashida
Jan 1, 1993·Alcohol and Alcoholism. Supplement·S Harada, T Okubo

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Citations

Sep 6, 2002·Cancer Letters·Pleumjit BoonyaphiphatPuttisak Puttawibul
Apr 23, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Robea BalloM Iqbal Parker
Nov 29, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Liang LiXiang-Min Xu
Aug 20, 2004·Alcoholism, Clinical and Experimental Research·Sakae ItogaFumio Nomura

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