Simultaneous HPLC-MS-MS quantification of 8-iso-PGF(2alpha) and 8,12-iso-iPF(2alpha) in CSF and brain tissue samples with on-line cleanup.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Magdalena KoreckaL M Shaw

Abstract

Quantitation of isoprostanes such as 8-iso-PGF(2alpha) and 8,12-iso-iPF(2alpha)-VI in biological fluids has been proposed as a reliable test of oxidant stress and inflammation in a variety of disorders. This paper presents a liquid chromatography method with tandem mass spectrometry detection for the simultaneous analysis of these two isoprostanes in human CSF and brain tissue samples. An API 5000 triple quadrupole instrument (AB Sciex, Foster City, CA, USA) with an APCI ion source was used in this study. Aliquots of CSF samples (0.25mL) were treated with a methanol:zinc sulfate mixture followed by on-line cleanup on an extraction column (Validated-C(18)) with 0.1% formic acid. The brain tissue samples were homogenized and lipids were extracted using Folch solution. Solid-phase extraction columns (C(18)) were used for the purification of the brain isoprostane fraction. Chromatographic separation was achieved using an analytical column (Synergi C(18) HydroRP) with 0.1% formic acid in water and a mixture of methanol:acetonitrile under isocratic conditions. The mass spectrometer was operated in the MRM scan and negative ion mode. The quadrupoles were set to detect the molecular ions [M-H](-) and high mass fragments of isoprostanes...Continue Reading

References

Nov 16, 2002·International Immunopharmacology·Kenneth L Hastings, UNKNOWN Center for Drug Evaluation and Research, US Food and Drug Administration
Nov 5, 2003·Journal of Mass Spectrometry : JMS·Cinzia SignoriniGianluca Giorgi
Jan 15, 2005·Antioxidants & Redox Signaling·Thomas J MontineJason D Morrow

❮ Previous
Next ❯

Citations

Nov 24, 2012·Bioanalysis·Y-J XueNaidong Weng
Mar 29, 2012·Bioanalysis·Kerri M Smith, Yan Xu
May 11, 2010·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Michael W WeinerUNKNOWN Alzheimer's Disease Neuroimaging Initiative
Apr 20, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dimitrios Tsikas, Alexander A Zoerner
May 27, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Claire VigorThierry Durand
Jul 4, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yi XiaoMaurice R O'Gorman
Jul 20, 2014·Drug and Alcohol Dependence·Timothy C DurazzoUNKNOWN Alzheimer's Disease Neuroimaging Initiative
Dec 10, 2014·Journal of Inherited Metabolic Disease·Benoit ColschChristophe Junot
Jul 1, 2020·Essays in Biochemistry·Omar S AhmedThierry Durand
Jul 30, 2016·Journal of Alzheimer's Disease : JAD·Timothy C DurazzoUNKNOWN Alzheimer’s Disease Neuroimaging Initiative
Sep 20, 2017·Progress in Lipid Research·Jean-Marie GalanoJetty Chung-Yung Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.