Simultaneous immunohistochemical demonstration of intra-axonally transported markers and neuropeptides in the peripheral nervous system of the guinea pig.

Histochemistry
B LindhT Hökfelt

Abstract

Projections and peptide neurotransmitter/neuromodulator content of autonomic and visceral afferent neurons of the guinea pig were studied after application of the subunit B of cholera toxin (CTB) with or without horseradish peroxidase (HRP) as retrograde and anterograde tracers and subsequent immunohistochemical processing for double staining using antibodies raised to CTB, HRP and various neuropeptides. The results demonstrate that substance P (SP)- and calcitonin gene-related peptide (CGRP)-containing dorsal root ganglion cells project to the pylorus as well as to the celiac superior mesenteric and stellate ganglia as demonstrated with both retrograde and anterograde transport methodology. Binding studies revealed that a small number of the CTB-binding dorsal root ganglion cells contains immunoreactivity to SP and CGRP. The majority of the CTB-binding cells is SP- and CGRP-negative and terminate in the deeper parts of the dorsal horn. After injection of CTB conjugated to HRP (B-HRP) into the nodose ganglion, both motor and sensory elements were labeled in the medulla oblongata. Some of the CTB labeled vagal sensory nerve fibers in the nucleus tractus solitarii (NTS) were also found to contain immunoreactivity to SP or CGRP. T...Continue Reading

References

Feb 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M M Mesulam
Dec 1, 1978·Acta Physiologica Scandinavica·J M LundbergS Said
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A C CuelloC Milstein
Oct 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M W Wessendorf, R P Elde
Jan 1, 1970·Acta Neuropathologica·K Kristensson
Jun 18, 1971·Brain Research·K Kristensson, Y Olsson
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·B K HartmanS Udenfriend
Jun 30, 1972·Science·J H LaVail, M M LaVail
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M R Matthews, A C Cuello
Aug 14, 1984·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M R Matthews, A C Cuello

❮ Previous
Next ❯

Citations

Jan 1, 1997·Pharmacology & Therapeutics·P Holzer, U Holzer-Petsche
May 2, 2001·The Journal of Comparative Neurology·R J Phillips, T L Powley
Aug 1, 2000·Archives of Physiology and Biochemistry·Z Pober
Aug 18, 2009·Autonomic Neuroscience : Basic & Clinical·Vladimir P ZagorodnyukNick J Spencer
Oct 30, 2012·Autonomic Neuroscience : Basic & Clinical·M ZaleckiJ Kaleczyc
Apr 16, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Kressel
Jan 16, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M I RomeroG M Smith
May 2, 2020·Chemical Communications : Chem Comm·Jessica L HaighW Bruce Turnbull
Feb 18, 2020·Brain Structure & Function·Jose L Lanciego, Floris G Wouterlood

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.