Simultaneous in situ detection of alkaline phosphatase activity and polyphosphate in arbuscules within arbuscular mycorrhizal roots

Functional Plant Biology : FPB
Rintaro FunamotoToshihiro Aono

Abstract

Inorganic phosphate (Pi) metabolism in arbuscules of arbuscular mycorrhizal (AM) fungi is not well understood, although recent research has revealed that host plants absorb Pi around arbuscules with mycorrhiza-specific transporters. Therefore, we analysed the localisation of polyphosphate (polyP) and alkaline phosphatase (ALP) activity in arbuscules, which could be indicators of Pi metabolism. We developed a dual-labelling method for polyP and ALP activity, i.e. first labelling with fluorescent probes 4',6-diamidino-2-phenyl-indole dihydrochloride (DAPI) and then labelling with enzyme-labelled fluorescence (ELF97). The dual-labelling method made it possible to observe polyP and ALP activity signals simultaneously in mycorrhizal roots. The dual-labelling method revealed that ALP activity was mainly observed in mature arbuscules where polyP was rarely observed. The expression of the AM fungal ALP gene was suppressed in the knockdown plants of an AM-inducible Pi-transporter, and there was much polyP in arbuscules that showed low ALP activity. These topological observations suggest that there may be some relationships between polyP metabolism and ALP activity in arbuscules, and that these are, in part, controlled by Pi uptake by pl...Continue Reading

References

Sep 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Uta PaszkowskiSteven P Briggs
Aug 9, 2003·Molecular Plant-microbe Interactions : MPMI·Hirotaka Kumagai, Hiroshi Kouchi
Aug 19, 2006·Canadian Journal of Microbiology·Katsuharu SaitoR Larry Peterson
Jan 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hélène JavotMaria J Harrison
Jul 1, 1997·The New Phytologist·Md Zakaria Solaimanand, Masanori Saito

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Citations

Aug 19, 2011·The Plant Journal : for Cell and Molecular Biology·Hélène JavotMaria J Harrison
Oct 8, 2019·Journal of Environmental Quality·Kimberley D SchneiderHenry F Wilson
Nov 11, 2016·The Journal of General and Applied Microbiology·Keiko KizawaRyo Ohtomo
Jan 13, 2011·FEMS Microbiology Ecology·Edith C HammerPål Axel Olsson
Dec 31, 2020·International Journal of Environmental Research and Public Health·Jessica A Rubin, Josef H Görres
Oct 8, 2021·Frontiers in Plant Science·Cuc Thi Nguyen, Katsuharu Saito

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