PMID: 9543416Apr 16, 1998Paper

Simultaneous intracellular and extracellular pH measurement in the heart by 19F NMR of 6-fluoropyridoxol

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
S HunjanP P Antich

Abstract

6-Fluoropyridoxol (6-FPOL) was evaluated as a simultaneous indicator of intracellular and extracellular pH and, hence, pH gradient in perfused rat hearts. After infusion, 19F NMR spectra rapidly showed two well-resolved peaks assigned to the intracellular and extracellular compartments, and pH was calculated on the basis of chemical shift with respect to a sodium trifluoroacetate standard. To demonstrate use of this molecule, dynamic changes in myocardial pH were assessed with a time resolution of 2 min during respiratory and metabolic alkalosis or acidosis and ischemia. For a typical heart, intracellular pH (pHi) = 7.14+/-0.01 and extracellular pH (pHe) = 7.52+/-0.02. In response to metabolic alkalosis, pHi remained relatively constant and the pH gradient increased. In contrast, respiratory challenge caused a significant increase in pHi. Independent measurements using pH electrodes and 31P NMR confirmed validity of the 19F NMR results.

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Citations

Sep 28, 2002·Journal of Magnetic Resonance Imaging : JMRI·Robert J GilliesZaver M Bhujwalla
Oct 24, 2001·Magnetic Resonance Imaging·K E LøklingJ Klaveness
Jul 25, 2003·Magnetic Resonance Imaging·Knut-Egil LøklingJo Klaveness
Jul 11, 2012·Journal of Medicinal Chemistry·Jian-Xin YuRalph P Mason
Mar 28, 2009·International Breastfeeding Journal·Mridula Bandyopadhyay
Feb 27, 2014·Magma·Anja Müller-LutzHans-Jörg Wittsack
Jun 21, 2014·Expert Opinion on Drug Delivery·Sara A AbouelmagdYoon Yeo
Dec 2, 2011·Biomaterials·Han Chang KangYou Han Bae
Aug 11, 2015·Chemical Communications : Chem Comm·Robert R FlavellDavid M Wilson
Jun 21, 2017·International Journal of Molecular Sciences·Tudor VasiliuLilia Clima
Apr 6, 2004·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Knut-Egil LøklingSigrid L Fossheim
Mar 17, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Sunil KapurGary L Aistrup
Mar 10, 2011·NMR in Biomedicine·Arig Ibrahim HashimRobert J Gillies
Nov 5, 2020·Chemical Communications : Chem Comm·Lili RenXin Zhou
Jan 9, 2021·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Changhua MuRobert R Flavell

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