Simultaneous LC-MS/MS determination of JWH-210, RCS-4, ∆(9)-tetrahydrocannabinol, and their main metabolites in pig and human serum, whole blood, and urine for comparing pharmacokinetic data

Analytical and Bioanalytical Chemistry
Nadine SchaeferPeter Schmidt

Abstract

A series of new synthetic cannabinoids (SC) has been consumed without any toxicological testing. For example, pharmacokinetic data have to be collected from forensic toxicological case work and/or animal studies. To develop a corresponding model for assessing such data, samples of controlled pig studies with two selected SC (JWH-210, RCS-4) and, as reference, ∆(9)-tetrahydrocannabinol (THC) should be analyzed as well as those of human cases. Therefore, a method for determination of JWH-210, RCS-4, THC, and their main metabolites in pig and human serum, whole blood, and urine samples is presented. Specimens were analyzed by liquid-chromatography tandem mass spectrometry and multiple-reaction monitoring with three transitions per compound. Full validation was carried out for the pig specimens and cross-validation for the human specimens concerning precision and bias. For the pig studies, the limits of detection were between 0.05 and 0.50 ng/mL in serum and whole blood and between 0.05 and 1.0 ng/mL in urine, the lower limits of quantification between 0.25 and 1.0 ng/mL in serum and 0.50 and 2.0 ng/mL in whole blood and urine, and the intra- and interday precision values lower than 15% and bias values within ±15%. The applicabilit...Continue Reading

References

Dec 28, 2002·Journal of Analytical Toxicology·Mark Hok Chi Chu, Olaf H Drummer
Jun 20, 2006·Forensic Science International : Synergy·Frank T PetersFrank Musshoff
Oct 5, 2007·Journal of Pharmaceutical and Biomedical Analysis·Aurélien ThomasChristian Staub
Oct 17, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maria Del Mar Ramirez FernandezNele Samyn
Jan 7, 2010·International Journal of Legal Medicine·Bertrand BrunetPatrick Mura
Apr 10, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jörg TeskeHans Dieter Tröger
Apr 24, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Andrew D de JagerAshley Hall
May 18, 2012·Journal of Analytical Toxicology·Julia AmmannJochen Beyer
Sep 20, 2012·The Journal of Emergency Medicine·Carson R Harris, Ashley Brown
Nov 17, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Enrique G Yanes, Dennis P Lovett
May 15, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marek DziadoszJörg Teske
May 23, 2013·Drug Testing and Analysis·Maren Hermanns-ClausenVolker Auwärter
Jan 29, 2014·Journal of Pharmaceutical and Biomedical Analysis·Camilla MontesanoRoberta Curini
Jul 23, 2014·Forensic Science International : Synergy·Nathalie J JaenickeStefan W Toennes

❮ Previous
Next ❯

Citations

Jan 15, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Michele ProttiLaura Mercolini
May 6, 2021·Journal of Analytical Toxicology·Frederike NordmeierNadine Schaefer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.