Simultaneous lipid production for biodiesel feedstock and decontamination of sago processing wastewater using Candida tropicalis ASY2

Biotechnology for Biofuels
Kiruthika ThangaveluSivakumar Uthandi

Abstract

Without sufficient alternatives to crude oil, as demand continues to rise, the global economy will undergo a drastic decline as oil prices explode. Dependence on crude oil and growing environmental impairment must eventually be overcome by creating a sustainable and profitable alternative based on renewable and accessible feedstock. One of the promising solutions for the current and near-future is the substitution of fossil fuels with sustainable liquid feedstock for biofuel production. Among the different renewable liquid feedstock's studied, wastewater is the least explored one for biodiesel production. Sago wastewater is the byproduct of the cassava processing industry and has starch content ranging from 4 to 7%. The present investigation was aimed to produce microbial lipids from oleaginous yeast, Candida tropicalis ASY2 for use as biodiesel feedstock and simultaneously decontaminate the sago processing wastewater for reuse. Initial screening of oleaginous yeast to find an efficient amylolytic with maximum lipid productivity resulted in a potent oleaginous yeast strain, C. tropicalis ASY2, that utilizes SWW as a substrate. Shake flask experiments are conducted over a fermentation time of 240 h to determine a suitable fatty ...Continue Reading

References

Dec 16, 2006·Journal of Industrial Microbiology & Biotechnology·Wei-Hua Chu
Sep 2, 2009·Progress in Lipid Research·Athanasios BeopoulosJean-Marc Nicaud
Dec 17, 2009·Journal of Hazardous Materials·Neha GuptaV K Agarwal
Apr 8, 2010·Bioresource Technology·Feiyan XueTianwei Tan
Jul 16, 2011·Bioresource Technology·Yeshitila Asteraye TsigieYi-Hsu Ju
Mar 7, 2014·Biotechnology for Biofuels·Fabio SantamauroChristopher J Chuck
Mar 19, 2014·Journal of Agricultural and Food Chemistry·Luis A GarayJ Bruce German
Feb 10, 2018·Applied Microbiology and Biotechnology·Marianna DourouGeorge Aggelis

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Methods Mentioned

BETA
Fluorescence
flow cytometry
bio-trap
electrophoresis
PCR

Software Mentioned

MEGA
BLAST
Excel

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