PMID: 9545112Apr 17, 1998Paper

Simultaneous measurement of free thyroxine and free 3,5,3'-triiodothyronine in undiluted serum by direct equilibrium dialysis/radioimmunoassay: evidence that free triiodothyronine and free thyroxine are normal in many patients with the low triiodothyronine syndrome

Thyroid : Official Journal of the American Thyroid Association
I J Chopra

Abstract

We have devised a practical, sensitive and specific method for simultaneous measurement of free thyroxine (FT4) and free triiodothyronine (FT3) in undiluted serum by direct equilibrium dialysis radioimmunoassay (RIA). Two hundred microliters serum sample was dialyzed against buffer (pH 7.4) for 20 hours at 37 degrees C and approximately 800 microL of the dialysate was used for measuring FT4 and FT3 simultaneously. The assay was set up in polystyrene tubes coated with anti-T4 antibody and available commercially for FT4 measurement (Quest-Nichols Institute, San Juan Capistrano, CA). The mean +/- SE (range) FT4 concentration (ng/dL) was 1.2 +/- 0.04 (0.7.0 to 2.30) in 54 normal subjects. It was significantly increased (3.6 +/- 0.4 [1.8 to 9.6], n = 20) in hyperthyroidism and clearly decreased (0.40 +/- 0.04 [1.10 to 0.70], n = 26] in hypothyroidism. All nonthyroid illness (NTI) patients had normal FT4 except 3, 2 of whom were on amiodarone and 1 had received heparin. Serum FT4 concentration was minimally elevated in 18 newborn cord blood serum (1.40 +/- 0.08 [0.90 to 2.2], cf. normal p < .05). The mean serum FT3 concentration (pg/dL) was 285 +/- 10 (134 to 454) in 54 normal sera. It was clearly increased in hyperthyroidism (1033 +...Continue Reading

References

Jul 1, 1975·The Journal of Clinical Endocrinology and Metabolism·F BermudezJ H Oppenheimer
Nov 1, 1988·The Journal of Clinical Endocrinology and Metabolism·M I SurksL E Shapiro
Oct 1, 1988·The Journal of Clinical Endocrinology and Metabolism·C F LimJ R Stockigt
Aug 1, 1987·The Journal of Clinical Endocrinology and Metabolism·J FaberI W Jensen
Sep 1, 1974·The Journal of Clinical Investigation·I J Chopra
Apr 1, 1974·The Journal of Clinical Endocrinology and Metabolism·C H Irvine
Jun 1, 1972·The Journal of Clinical Endocrinology and Metabolism·S W Spaulding, R I Gregerman
Jun 1, 1981·The Journal of Clinical Endocrinology and Metabolism·E M KapteinJ T Nicoloff
Nov 1, 1981·The Journal of Clinical Endocrinology and Metabolism·S MelmedJ M Hershman
Aug 1, 1996·Thyroid : Official Journal of the American Thyroid Association·I J ChopraL Mikus
Feb 1, 1997·Thyroid : Official Journal of the American Thyroid Association·V Jeevanandam

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Citations

Jun 12, 2002·Endocrinology and Metabolism Clinics of North America·Joanne E Langton, Gregory A Brent
Apr 6, 2006·Journal of Veterinary Internal Medicine·Babetta A BreuhausSusan L Beyerlein
Oct 4, 2006·The Journal of Clinical Investigation·Antonio C Bianco, Brian W Kim
Aug 31, 2011·Journal of Thyroid Research·Petra MohácsikBalázs Gereben
Sep 29, 2007·Critical Care Clinics·Olga V Sakharova, Silvio E Inzucchi
Aug 4, 2007·Endocrinology and Metabolism Clinics of North America·Suzanne Myers Adler, Leonard Wartofsky
Feb 24, 2001·Critical Care Clinics·K D Burman, L Wartofsky
Jul 27, 2002·Critical Care Clinics·Jeffrey I Mechanick, Elise M Brett
Nov 1, 2005·Physiology & Behavior·Dana L HelmreichDavid B Parfitt
May 14, 1999·The Journal of Clinical Endocrinology and Metabolism·L WartofskyM D Ringel
Dec 5, 2019·Thyroid : Official Journal of the American Thyroid Association·Jerome HershmanPeter A Kopp
Mar 17, 2007·Clinical Chemistry·Kristofer S FritzJerald C Nelson
Jan 27, 1999·The Journal of Clinical Endocrinology and Metabolism·L J De Groot
Apr 2, 2004·Journal of Endocrinological Investigation·N Stathatos, L Wartofsky
May 18, 2013·Arquivos brasileiros de endocrinologia e metabologia·Gisah Amaral de CarvalhoLaura Sterian Ward

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