Simultaneous measurement of intracellular Ca2+ and asynchronous transmitter release from the same crayfish bouton

The Journal of Physiology
R RavinI Parnas

Abstract

1. A technique has been developed to monitor neurotransmitter release simultaneously with intracellular Ca2+ concentration ([Ca2+]i) in single release boutons whose diameters range from 3 to 5 microns. 2. Using this technique, we have found a highly non-linear relationship between the rate of asynchronous release and [Ca2+]i. The Hill coefficient lies between 3 and 4. 3. The affinity (Kd) of the putative release-related Ca2+ receptor for asynchronous release was calculated to be in the range of 2-4 microM. 4. The same range of values of Hill coefficient and Kd were obtained when [Ca2+]i was elevated both by bath application of ionomycin and by repetitive stimulation at high frequency. 5. Our results show that the Ca2+ receptor(s) associated with asynchronous release exhibits high affinity for Ca2+.

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Citations

Feb 5, 2002·Annual Review of Physiology·Robert S Zucker, Wade G Regehr
Jan 13, 2011·Neural Computation·Vladislav Volman, Richard C Gerkin
Nov 30, 2007·Nature·Jianyuan SunThomas C Südhof
Aug 28, 2004·Journal of Neurophysiology·Remigijus Lape, John A Dani
Feb 8, 2005·The Journal of Physiology·Ron L P Habets, J Gerard G Borst
Aug 1, 2007·Physical Biology·Vladislav VolmanGuo-Qiang Bi
Sep 3, 2010·Pflügers Archiv : European journal of physiology·Itzchak Parnas, Hanna Parnas
Sep 17, 2005·The Journal of Physiology·Eve-Lyne BélairRichard Robitaille
Jul 23, 2008·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Vladislav Volman, Herbert Levine
Jun 27, 2014·Journal of Neurophysiology·Jessica L HauserChinfei Chen
Mar 10, 2000·Journal of Applied Physiology·M E Crider, R L Cooper
May 30, 2009·Journal of Neurochemistry·Shengzhou WuB Joy Snider
Jan 12, 2001·Journal of Neurophysiology·J K Angleson, W J Betz
Jun 5, 2003·Journal of Neurophysiology·Nidhi Rumpal, Gregory A Lnenicka
Feb 26, 2003·Cell Calcium·Gavriel DavidEllen F Barrett
Feb 27, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Sana SharifianKhosro Khajeh

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