Simultaneous measurement of ionic current and fluorescence from single protein pores.

Journal of the American Chemical Society
Andrew HeronMark I Wallace

Abstract

The ability to simultaneously monitor both the ionic current and fluorescence from membrane channels and pores has the potential to link structural changes with function in such proteins. We present a new method for simultaneously measuring single-channel electrical currents and fluorescence from membrane proteins by using water-in-oil droplet bilayers. We demonstrate the simultaneous fluorescence and electrical detection of stochastic blocking by cyclodextrin in multiple staphylococcal alpha-hemolysin pores. The combined fluorescence signal from individual pores exhibits the same sequence of blocking events as the total current recording, showing that the two signals from each pore are correlated.

References

Jan 1, 1995·Progress in Biophysics and Molecular Biology·A G MacDonald, P C Wraight
Oct 6, 1998·The Journal of General Physiology·E Loots, E Y Isacoff
Oct 19, 2001·Biophysical Journal·G S HarmsT Schmidt
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Hui ZouJoshua J Singer
Jan 14, 2003·Biophysical Journal·V BorisenkoG J Schütz
Feb 27, 2007·Nature·Christoph BiskupKlaus Benndorf
Jun 19, 2007·Journal of the American Chemical Society·Matthew A HoldenHagan Bayley
Jun 19, 2007·Bioorganic & Medicinal Chemistry·Vladimir A KarginovSidney M Hecht
Dec 7, 2007·Journal of the American Chemical Society·Andrew J HeronMark I Wallace
Mar 21, 2008·Nature Reviews. Molecular Cell Biology·Francisco Bezanilla
Apr 17, 2008·Annual Review of Biochemistry·Chirlmin JooTaekjip Ha
Apr 28, 2009·Molecular BioSystems·Hagan BayleyMark Wallace

❮ Previous
Next ❯

Citations

Oct 8, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Linda C M GrossMark I Wallace
Nov 8, 2011·Nature Nanotechnology·Gabriel VillarHagan Bayley
May 4, 2013·Nature Protocols·Sebastian LeptihnMark I Wallace
Jul 16, 2014·Journal of Molecular Biology·Eve E Weatherill, Mark I Wallace
Sep 10, 2015·Biochimica Et Biophysica Acta·Nejc RojkoGregor Anderluh
Jul 24, 2013·Biophysical Chemistry·Marta MarchiorettoGregor Anderluh
Sep 1, 2015·Nature Nanotechnology·Shuo HuangMark I Wallace
Aug 2, 2015·Chemistry and Physics of Lipids·C Patrick Collier
Sep 17, 2015·Biochimica Et Biophysica Acta·Katia CosentinoAna J García-Sáez
Jul 27, 2015·Biochimica Et Biophysica Acta·Marc Szabo, Mark I Wallace
Jan 21, 2012·Biophysical Journal·James R ThompsonMark I Wallace
Dec 17, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Graham J Taylor, Stephen A Sarles
Dec 14, 2016·Analytical Chemistry·Toshihisa Osaki, Shoji Takeuchi
Jun 8, 2017·Biophysical Journal·Tatyana I RokitskayaYuri N Antonenko
Feb 7, 2017·Soft Matter·John S H DanialMark I Wallace
Feb 17, 2015·The Analyst·Tal Gilboa, Amit Meller
Nov 2, 2017·Chemical Communications : Chem Comm·Julia M ThomasYuval Elani
Aug 3, 2017·Molecular BioSystems·Michael J BoothHagan Bayley
Oct 13, 2018·Chemical Communications : Chem Comm·Maie A ElfaramawyTomoaki Matsuura
Sep 11, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Toru Ide
Feb 9, 2016·Scientific Reports·Robert R IshmukhametovRichard M Berry
Dec 6, 2014·Clinical Chemistry·Hagan Bayley
Sep 17, 2019·Science Advances·Yuqin WangShuo Huang
Nov 16, 2012·Scientific Reports·K Tanuj Sapra, Hagan Bayley
Aug 6, 2010·Physical Biology·Johannes HohlbeinAchillefs N Kapanidis
Jan 27, 2011·The Analyst·Sophie DemarcheJanos Vörös
Apr 30, 2014·Angewandte Chemie·Tiandi Zhuang, Lukas K Tamm
Aug 30, 2012·Angewandte Chemie·Arnold J Boersma, Hagan Bayley
Sep 7, 2019·Physical Review Letters·M ValetA M Prevost
May 5, 2021·Current Opinion in Structural Biology·Uris RosAna J Garcia-Saez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.