PMID: 1202193Oct 1, 1975Paper

Simultaneous measurements of magnesium, calcium and sodium influxes in perfused squid giant axons under membrane potential control

The Journal of Physiology
E Rojas, R E Taylor

Abstract

1. Giant axons from the squids Dosidicus gigas, Loligo forbesi and Loligo vulgaris were internally perfused with 550 or 275 mM KF plus sucrose and bathed in artificial sea water containing 45Ca, 28Mg or mixtures of 45Ca-28Mg or 45Ca-22Na. Resting influxes and extra influxes during voltage-clamp pulses were measured by collecting and counting the internal perfusate. 2. For Dosidicus axons in 10 mM-CaCl2 the resting influx of calcium was 0-016 +/- 0-007 p-mole/cm2 sec and a linear function of external concentration. For two experiments in 10 and 84-7 mM-CaCl2, 100 nM tetrodotoxin had no effect. Resting calcium influx in 10 mM-CaCl2 was 0-017 +/- 0-013 p-mole/cm2 sec for Loligo axons. 3. With 55 mM-MgCl2 outside the average resting magnesium influx was 0-124 +/- 0-080 p-mole/cm2 sec for Loligo axons. Discarding one aberrant point the value is 0-105 +/- 0-046 which is not significantly different from the resting calcium influx for Dosidicus fibres in 55 mM-CaCl2, given as 0-094 p-mole/cm2 sec by the regression line shown in Fig. 1. In two experiments 150 nM tetrodotoxin had no effect. 4. With 430 mM-NaCl outside 100 nM tetrodotoxin reduced the average resting influx of sodium in Dosidicus axon from 27-7 +/- 4-5 to 25-1 +/- 6-2 p-mo...Continue Reading

Citations

Mar 3, 1978·Journal of Mathematical Biology·E Siep
Oct 19, 1978·The Journal of Membrane Biology·L J Mullins, F J Brinley
Jan 6, 1983·Nature·J C HenquinM Cogneau
Aug 1, 1979·Quarterly Reviews of Biophysics·J Requena, L J Mullins
Jan 1, 1984·The Journal of Membrane Biology·P W Flatman
Apr 10, 2014·Bioinspiration & Biomimetics·A G WinterA E Hosoi
Sep 1, 1977·CRC Critical Reviews in Microbiology·R M Atlas
Apr 23, 1987·Biochimica Et Biophysica Acta·E Heinonen, K E Akerman
Jun 1, 1982·Neuroscience·C Edwards
Feb 7, 1977·Biochemical and Biophysical Research Communications·T Maruyama, H Ishikawa
May 29, 2007·Experimental and Molecular Pathology·D Radford Shanklin

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