Simultaneous microstructural and mechanical characterization of human corneas at increasing pressure

Journal of the Mechanical Behavior of Biomedical Materials
Aurélie BenoitJean-Marc Allain

Abstract

The cornea, through its shape, is the main contributor to the eye׳s focusing power. Pathological alterations of the cornea strongly affect the eye power. To improve treatments, complex biomechanical models have been developed based on the architecture and mechanical properties of the collagen network in the stroma, the main layer of the cornea. However, direct investigations of the structure of the stroma, as well as its link to the mechanical response, remained limited. We propose here an original set up, associating nonlinear optical imaging and mechanical testing. By using polarization resolved Second Harmonic signals, we simultaneously quantified micrometer (orientation of the collagen lamellae) and nanometer (local disorder within lamellae) scale corneal organization. We showed that the organization of the lamellae changes along the stroma thickness. Then, we measured simultaneously the deformation on the epithelial side of the cornea and the reorientation of the collagen lamellae for increasing intraocular pressure levels, from physiological ones to pathological ones. We showed that the observed deformation is not correlated to initial orientation, but to the reorganization of the lamellae in the stroma. Our results, by p...Continue Reading

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Citations

Mar 31, 2017·Biomechanics and Modeling in Mechanobiology·C JayyosiM Coret
Apr 5, 2018·Journal of Cataract and Refractive Surgery·Joaquín FernándezDavid P Piñero
Aug 10, 2017·Optics Express·Lukas KontenisVirginijus Barzda
Oct 21, 2017·Scientific Reports·Kate GrieveVincent Borderie
Oct 25, 2017·Scientific Reports·Barbara LynchMarie-Claire Schanne-Klein
Sep 13, 2019·Optics Express·Margaux SchmeltzMarie-Claire Schanne-Klein
Feb 6, 2020·Optometry and Vision Science : Official Publication of the American Academy of Optometry·D Robert IskanderPatrycja Krzyżanowska-Berkowska
Mar 5, 2020·Applied Physics Letters·John J PitreIvan Pelivanov
May 23, 2021·Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)·Marcelina SobczakBarbara Pierścionek
May 19, 2021·Translational Vision Science & Technology·Monika E DanielewskaD Robert Iskander
Aug 31, 2021·Biomedical Optics Express·Clothilde RaouxMarie-Claire Schanne-Klein

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