Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat

The Plant Journal : for Cell and Molecular Biology
Yunwei ZhangDingzhong Tang

Abstract

Wheat (Triticum aestivum L.) incurs significant yield losses from powdery mildew, a major fungal disease caused by Blumeria graminis f. sp. tritici (Bgt). enhanced disease resistance1 (EDR1) plays a negative role in the defense response against powdery mildew in Arabidopsis thaliana; however, the edr1 mutant does not show constitutively activated defense responses. This makes EDR1 an ideal target for approaches using new genome-editing tools to improve resistance to powdery mildew. We cloned TaEDR1 from hexaploid wheat and found high similarity among the three homoeologs of EDR1. Knock-down of TaEDR1 by virus-induced gene silencing or RNA interference enhanced resistance to powdery mildew, indicating that TaEDR1 negatively regulates powdery mildew resistance in wheat. We used CRISPR/Cas9 technology to generate Taedr1 wheat plants by simultaneous modification of the three homoeologs of wheat EDR1. No off-target mutations were detected in the Taedr1 mutant plants. The Taedr1 plants were resistant to powdery mildew and did not show mildew-induced cell death. Our study represents the successful generation of a potentially valuable trait using genome-editing technology in wheat and provides germplasm for disease resistance breeding.

References

Jun 23, 1998·The Plant Cell·C A Frye, R W Innes
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·D HoisingtonM Warburton
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Steve HolzbergGregory P Pogue
Feb 6, 2004·The Plant Journal : for Cell and Molecular Biology·Nabila YahiaouiBeat Keller
Dec 8, 2004·Nature Biotechnology·Ann J SladeDaniel Facciotti
Feb 22, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ronghua ZhouJizeng Jia
Sep 9, 2005·DNA Sequence : the Journal of DNA Sequencing and Mapping·Feng-Shan LiangBin Wang
Mar 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marieke van HultenJurriaan Ton
Nov 1, 2006·Molecular Plant Pathology·Matt HumphryRalph Panstruga
Sep 3, 2010·Plant, Cell & Environment·Xiangling ShenShiping Wang
Mar 29, 2011·Plant Biotechnology Journal·Susanne BrunnerBeat Keller
Apr 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Aizhong CaoPeidu Chen
Sep 14, 2011·Annual Review of Genetics·Lorraine S Symington, Jean Gautier
Nov 1, 2011·The Plant Journal : for Cell and Molecular Biology·Huairong PanDingzhong Tang
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Jan 5, 2013·Molecular Plant·Qiwei ShanCaixia Gao
Mar 5, 2013·Annual Review of Plant Biology·Daniel F Voytas
Mar 29, 2013·Nature·Hong-Qing LingJun Wang
Aug 10, 2013·Nature Biotechnology·Qiwei ShanCaixia Gao
Oct 15, 2013·G3 : Genes - Genomes - Genetics·Santosh Kumar UpadhyayRakesh Tuli
Oct 16, 2013·The Plant Journal : for Cell and Molecular Biology·Severine HurniBeat Keller
Mar 1, 2014·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Zhen LiangCaixia Gao
Aug 5, 2014·Trends in Biochemical Sciences·Youssef BelkhadirJoanne Chory
Sep 19, 2014·Nature Protocols·Qiwei ShanCaixia Gao
Oct 4, 2014·Trends in Plant Science·Rosa Lozano-Durán, Cyril Zipfel
Jan 21, 2015·Plant Biotechnology Journal·Qiwei ShanCaixia Gao
Mar 2, 2016·International Journal of Molecular Sciences·Unnikrishnan UnniyampurathManoj N Krishnan
Jun 15, 2016·Annual Review of Phytopathology·Ravi P SinghJulio Huerta-Espino
Aug 28, 2016·Plant Biotechnology Journal·Johanna Acevedo-GarciaRalph Panstruga

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Citations

Jun 13, 2018·Biotechnology & Genetic Engineering Reviews·Reagan MudziwapasiMcebisi Maphosa
Jul 6, 2018·Annual Review of Phytopathology·Thorsten LangnerKhaoula Belhaj
Sep 7, 2018·Nature Biotechnology·Fyodor D UrnovDana Carroll
Oct 31, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jian XiaYueming Yan
Nov 6, 2018·Journal of Integrative Plant Biology·Jochen KumlehnHolger Puchta
Jan 16, 2018·Plant Cell Reports·Yuhei KanazashiTetsuya Yamada
Dec 7, 2018·Genome Biology·Yi ZhangCaixia Gao
Jan 17, 2019·Critical Reviews in Biotechnology·Aili BaoLam-Son Phan Tran
Jul 19, 2019·Journal of Cellular Physiology·Niaz AhmadBaohong Zhang
Aug 21, 2019·Microorganisms·Rahul Mahadev ShelakeJae-Yean Kim
Oct 28, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ke WangXingguo Ye
Dec 28, 2017·The New Phytologist·Shenghao ZouDingzhong Tang
Aug 28, 2020·Frontiers in Plant Science·Kamal KhadkaAlireza Navabi
Sep 26, 2020·Nature Reviews. Molecular Cell Biology·Haocheng ZhuCaixia Gao
Sep 8, 2017·Frontiers in Plant Science·Muhammad N SattarSuliman A Al-Khateeb
Apr 9, 2019·BioMed Research International·Nikolai BorisjukYuri Shavrukov
Aug 23, 2019·International Journal of Molecular Sciences·Ali RazzaqFaiz Ahmad Joyia
Mar 21, 2019·Frontiers in Bioengineering and Biotechnology·Michael F EckerstorferFriedrich Waßmann
Jun 11, 2020·International Journal of Molecular Sciences·Waquar A AnsariRupesh Deshmukh
Jul 21, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Shijin LiXingguo Ye
Sep 11, 2018·Frontiers in Plant Science·Virginia M G BorrelliAlessandra Lanubile
Oct 5, 2018·BMC Plant Biology·Rhian M HowellsEmma J Wallington
Mar 3, 2020·Frontiers in Plant Science·Kaoutar El-MounadiHernan Garcia-Ruiz
Aug 15, 2020·Frontiers in Plant Science·Erika N DortRichard C Hamelin
Aug 14, 2020·International Journal of Molecular Sciences·Sunny AhmarKi-Hong Jung
Apr 4, 2019·Molecular Biology Reports·Rakesh KumarG P Singh
Jan 10, 2020·Briefings in Functional Genomics·Shakeel AhmadShaoqing Tang
May 31, 2019·International Journal of Molecular Sciences·Iris KoeppelJochen Kumlehn
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Kangquan Yin, Jin-Long Qiu

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