Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea

The Korean Journal of Parasitology
Seobo SimJae-Ran Yu

Abstract

Cryptosporidium and Cyclospora are well-known coccidian protozoa that can cause waterborne and foodborne diarrheal illnesses. There have been a few reports regarding contamination in different vegetables with Cryptosporidium, but no data are available regarding the sources of Cyclospora infections in Korea. In the present study, we collected 6 kinds of vegetables (perilla leaves, winter-grown cabbages, chives, sprouts, blueberries, and cherry tomatoes) from July 2014 to June 2015, and investigated contamination by these 2 protozoa using multiplex quantitative real-time PCR. Among 404 vegetables, Cryptosporidium and Cyclospora were detected in 31 (7.7%) and 5 (1.2%) samples, respectively. In addition, Cryptosporidium was isolated from all 6 kinds of vegetables, whereas Cyclospora was detected in 4 kinds of vegetables (except perilla leaves and chives). Cryptosporidium (17.8%) and Cyclospora (2.9%) had the highest detection rates in chives and winter-grown cabbages, respectively. Cryptosporidium was detected all year long; however, Cyclospora was detected only from October to January. In 2 samples (sprout and blueberry), both Cryptosporidium and Cyclospora were detected. Further investigations using TaqI restriction enzyme fragme...Continue Reading

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Citations

Jul 31, 2020·Parasites & Vectors·Junqiang LiLongxian Zhang
Sep 7, 2019·Microorganisms·Sonia AlmeriaJitender P Dubey
Dec 15, 2020·Journal of Environmental Health Science & Engineering·Ehsan JavanmardPanagiotis Karanis
Sep 26, 2020·Clinical and Experimental Pediatrics·Eell Ryoo

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

SDS
Clone Manager
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