PMID: 8602343Dec 1, 1995Paper

Simultaneous monitoring of intracellular pH and proton excretion during glycolysis by Streptococcus mutans and Streptococcus sanguis: effect of low pH and fluoride

Oral Microbiology and Immunology
Y IwamiT Yamada

Abstract

A system was developed by which 2',7'-bis(carboxyethyl)-4 or 5-carboxyfluorescein could be used to monitor intracellular pH at the same time that proton excretion was being measured. Streptococcal cells were loaded with the dye, and after the addition of glucose protons were excreted and the intracellular pH increased quickly and remained higher than the extracellular pH of 7.0. The excretion of protons stopped and the intracellular pH returned to the original level when glucose was depleted. The intracellular level of ATP remained high during glucose metabolism and decreased with the depletion of glucose. At extracellular pH of 5.5, and 5.0, the intracellular pH of fasting cells was higher than the extracellular pH value. After addition of glucose there were initial lags of proton excretion and of increases in intracellular pH at the acidic extracellular pH values. In the presence of fluoride, a lag in proton excretion and a simultaneous decrease in intracellular pH were observed, indicating a partial and transient inhibition of proton-ATPase activity.

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Citations

Feb 15, 2003·FEMS Microbiology Reviews·Robert E MarquisMarilaine Mota-Meira
Feb 10, 2004·Applied and Environmental Microbiology·Elizabeth M Fozo, Robert G Quivey
Feb 12, 2010·Immunopharmacology and Immunotoxicology·Tsung C Kuo
Jun 26, 2003·Oral Microbiology and Immunology·H MiyasawaN Takahashi
Apr 1, 1997·Advances in Dental Research·J Carlsson
Feb 18, 2009·Clinical Implant Dentistry and Related Research·José Henrique Rubo, Edson Antonio Capello Souza
Nov 3, 2009·The Journal of Craniofacial Surgery·Erica Alves GomesEdson Antonio Capello de Sousa
May 21, 2010·The Journal of Craniofacial Surgery·Eduardo Piza PellizzerHumberto Gennari Filho
May 21, 2010·The Journal of Craniofacial Surgery·Wirley Gonçalves AssunçãoEdson Antonio Capello de Sousa
Jun 16, 2010·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Ricardo T AbreuMarcelo F Mesquita
Nov 23, 2012·The Journal of Craniofacial Surgery·Marcelo Bighetti ToniolloMaria da Gloria Chiarello de Mattos
Oct 3, 2019·Chemical Research in Toxicology·Nichole R Johnston, Scott A Strobel

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