PMID: 11607137Dec 15, 1990Paper

Simultaneous monitoring of light-induced changes in protein side-group protonation, chromophore isomerization, and backbone motion of bacteriorhodopsin by time-resolved Fourier-transform infrared spectroscopy

Proceedings of the National Academy of Sciences of the United States of America
K GerwertB Hess

Abstract

Absorbance changes in the infrared and visible spectral range were measured in parallel during the photocycle of light-adapted bacteriorhodopsin, which is accompanied by a vectorial proton transfer. A global fit analysis yielded the same rate constants for the chromophore reactions, for protonation changes of protein side groups, and for the backbone motion. From this result we conclude that all reactions in various parts of the protein are synchronized to each other and that no independent cycles exist for different parts. The carbonyl vibration of Asp-85, indicating its protonation, appears with the same rate constant as the Schiff base deprotonation. The carbonyl vibration of Asp-96 disappears, indicating most likely its deprotonation, with the same rate constant as for the Schiff base reprotonation. This result supports the proposed mechanism in which the protonated Schiff base, a deprotonated aspartic acid (Asp-85) on the proton-release pathway, and a protonated aspartic acid (Asp-96) on the proton-uptake pathway act as internal catalytic proton-binding sites.

References

Sep 15, 1978·FEBS Letters·R KorensteinD Kuschmitz
Sep 1, 1975·Biophysical Journal·R H LozierW Stoeckenius
Jun 20, 1990·Journal of Molecular Biology·R HendersonK H Downing
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K GerwertD Oesterhelt
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H OttoM P Heyn
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K J RothschildJ Herzfeld
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M HolzH G Khorana
Dec 1, 1989·Photochemistry and Photobiology·T Kitagawa, A Maeda
May 1, 1989·Photochemistry and Photobiology·D S ChernavskiiB V Zubov
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M S BraimanK J Rothschild
Apr 1, 1987·Biophysical Journal·A H XieR H Lozier
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·D Oesterhelt, W Stoeckenius
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J H HanamotoM A El-Sayed
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Z DancsházyT G Ebrey

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Citations

Jan 1, 1993·Photosynthesis Research·J Lavergne, W Junge
Apr 1, 1992·Journal of Bioenergetics and Biomembranes·K J Rothschild
Apr 1, 1992·Journal of Bioenergetics and Biomembranes·J K Lanyi
Apr 1, 1992·Journal of Bioenergetics and Biomembranes·D OesterheltE Bamberg
Sep 1, 1995·Biophysical Chemistry·H J SteinhoffW L Hubbell
Sep 1, 1995·Biophysical Chemistry·J K Lanyi
Sep 12, 1998·Biochimica Et Biophysica Acta·J FidyM Köhler
Jul 7, 1999·Biochimica Et Biophysica Acta·E GoormaghtighJ M Ruysschaert
Jun 6, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·J PajakL Sobczyk
Feb 1, 1993·Quarterly Reviews of Biophysics·E BambergK Fendler
Apr 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Carsten KöttingKlaus Gerwert
Dec 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Prasad PhatakMarcus Elstner
Jun 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Erik FreierKlaus Gerwert
Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Konstantin GavriljukKlaus Gerwert
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A DérW Stoeckenius
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Heberle, N A Dencher
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·U HauptsD Oesterhelt
Jun 1, 1992·Journal of Biomolecular Structure & Dynamics·R Sankararamakrishnan, S Vishveshwara
Apr 26, 2006·Photochemistry and Photobiology·Akio MaedaThomas G Ebrey
Mar 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Florian GarczarekKlaus Gerwert
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·C AllinK Gerwert
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·L S BrownJ K Lanyi
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·E KauffmannK Gerwert
Dec 5, 2006·Journal of Molecular Biology·Janos K Lanyi, Brigitte Schobert
Nov 5, 2015·Biophysical Journal·Tsung-Yen LeeTsyr-Yan Yu
Nov 4, 1994·Journal of Molecular Biology·M KataokaJ K Lanyi
Aug 19, 2007·Biochimica Et Biophysica Acta·Andreas Barth
Sep 24, 2013·Biochimica Et Biophysica Acta·Klaus GerwertSteffen Wolf
Mar 1, 2014·Journal of Structural Biology·Coral del ValAna-Nicoleta Bondar
Jul 6, 2014·Biophysical Journal·Steffen WolfKlaus Gerwert
Feb 16, 2007·Journal of Molecular Biology·Partha P ChakrabartiAlfred Wittinghofer

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