Simultaneous multi-parameter observation of Harringtonine-treating HL-60 cells with both two-photon and confocal laser scanning microscopy

Science in China. Series C, Life Sciences
C ZhangD Chen

Abstract

Harringtonine (HT), a kind of anticancer drug isolated from Chinese herb-Cephalotaxus hainanensis Li, can induce apoptosis in promyelocytic leukemia HL-60 cells. With both two-photon laser scanning microscopy and confocal laser scanning microscopy in combination with the fluorescent probe Hoechst 33342, tetramethyrhodamine ethyl ester (TMRE) and Fluo 3-AM, we simultaneously observed HT-induced changes in nuclear morphology, mitochondrial membrane potential and intracellular calcium concentration ([Ca(2+)](i)) in HL-60 cells, and developed a real-time, sensitive and invasive method for simultaneous multi-parameter observation of drug-treating living cells at the level of single cell.

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Citations

Jul 12, 2005·American Journal of Medical Genetics. Part a·Grétel G OudesluijsRaoul C M Hennekam
Apr 2, 2008·European Biophysics Journal : EBJ·I TestaA Diaspro
Jun 28, 2003·Natural Product Reports·Kenneth W Bentley
May 5, 2021·Chinese Journal of Natural Medicines·Da-Cheng HaoGuang-Bo Ge

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis