Simultaneous multi-site two-photon photostimulation in three dimensions

Journal of Biophotonics
Mary Ann GoVincent R Daria

Abstract

We demonstrate simultaneous multi-site two-photon photolysis of caged neurotransmitters with close to diffraction-limited resolution in all three dimensions (3D). We use holographic projection of multiple focal spots, which allows full control over the 3D positions of uncaging sites with a high degree of localized excitation. Our system incorporates a two-photon imaging setup to visualize the 3D morphology of the neurons in order to accurately determine the photostimulation sites. We show its application to studies of synaptic integration by performing simultaneous and controlled glutamate delivery at multiple locations on dendritic trees.

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Citations

Jun 21, 2013·Biology of the Cell·Eirini Papagiakoumou
Dec 17, 2014·Journal of Neuroscience Methods·Serena Bovetti, Tommaso Fellin
May 27, 2014·Frontiers in Cellular Neuroscience·Sunggu YangCha-Min Tang
Jan 8, 2016·Scientific Reports·Mary Ann GoVincent Ricardo Daria
Dec 27, 2016·Biomedical Optics Express·Michael Lawrence CastanaresVincent R Daria
Jan 9, 2019·Frontiers in Cellular Neuroscience·Emiliano RonzittiEirini Papagiakoumou
Feb 28, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tina ZhangAntonio Tricoli
Mar 26, 2014·Optics Express·Andrew BañasJesper Glückstad
Oct 28, 2015·Biomedical Optics Express·Vini GautamVincent R Daria
Apr 21, 2017·Open Biology·Ehsan KheradpezhouhEhsan Arabzadeh
Mar 23, 2021·Biophysics Reviews·Vincent R DariaHans-A Bachor

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