Simultaneous multichannel nonlinear imaging: combined two-photon excited fluorescence and second-harmonic generation microscopy

Micron : the International Research and Review Journal for Microscopy
R GauderonC J Sheppard

Abstract

Simultaneous two-photon excited fluorescence (TPF) and second-harmonic generation (SHG) imaging is demonstrated using a single femtosecond laser and a scanning microscope. This composite nonlinear microscopic technique was applied to imaging DNA and chromosomes, and it was shown that the two different interaction mechanisms provide complementary information on the structure and nonlinear properties of these biological materials, beyond that achievable using either TPF or SHG imaging alone. The use of separate modes of detection, in reflection and transmission respectively, and the simultaneous nature of the acquisition of the two images allows pure TPF and SHG images in precise registration to be obtained.

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Citations

Jun 28, 2012·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·C HussK König
May 4, 2001·Micron : the International Research and Review Journal for Microscopy·R GauderonC J Sheppard
May 3, 2011·Journal of Biomedical Optics·Shulian WuShufei Xu
Jul 15, 2004·Journal of Biomedical Optics·Meng HanJosef F Bille
Aug 31, 2004·Lasers in Surgery and Medicine·Petra Wilder-SmithTatiana Krasieva
Jan 28, 2009·Cellular Microbiology·Vjollca Konjufca, Mark J Miller
Jun 23, 2016·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Shana R Watson, Susan M Lessner
Jan 12, 2017·Scientific Reports·Joanna L NgMelissa L Knothe Tate
Jan 18, 2006·Journal of Dentistry·Paulo H P D'AlpinoDavid H Pashley
Aug 3, 2007·Physics in Medicine and Biology·Shuangmu ZhuoShuyuan Xiong
Jan 24, 2020·Journal of Biomedical Optics·Colin J R Sheppard
Jan 30, 2008·Current Protocols in Cell Biology·Peter FriedlGregory Harms

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