Simultaneous, Multiplexed Detection of RNA and Protein on the NanoString® nCounter® Platform

Methods in Molecular Biology
Sarah Warren

Abstract

The NanoString nCounter Analysis System uses a digital fluorescent barcode technology that allows for direct multiplexed measurement of gene expression (mRNA), DNA, and protein. The technology uses molecular barcodes and single-molecule imaging to detect and count unique mRNA and protein targets in a single reaction. nCounter-based detection is enzyme-free (no amplification of mRNA is required), fully automated, and allows simultaneous detection of up to 770 mRNA and 30 protein targets from multiple sample types. Target counting is fully digital with quantitative data output. Here we describe preparation of solid tumor lysate samples for use in the nCounter Analysis System.

Citations

Sep 24, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Carl Robert RankinDavid Miguel Padua
Aug 6, 2020·International Journal of Molecular Sciences·Ti-Hui WuYa-Wen Lin
Jul 31, 2019·Genome Medicine·Noel F C C de Miranda, Zlatko Trajanoski
Feb 14, 2021·Scientific Reports·Jillian W P BrachtMiguel A Molina-Vila
Aug 10, 2021·Expert Opinion on Investigational Drugs·Berta CaballolAzucena Salas

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