PMID: 3320819Nov 23, 1987Paper

Simultaneous observation of fluorescent retrogradely labeled neurons and the immunofluorescently labeled fibers apposing them using Fluoro-Gold and antisera labeled with the blue fluorochrome 7-amino-4-methylcoumarin-3-acetic acid (AMCA)

Neuroscience Letters
M W WessendorfR P Elde

Abstract

In this paper we describe a method allowing the simultaneous observation of fluorescent retrogradely labeled neurons and the immunofluorescently labeled fibers which appose them. This technique employs the fluorescent dye Fluoro-Gold to label neurons retrogradely, and 7-amino-4-methylcoumarin-3-acetic-acid (AMCA) conjugated to a secondary antiserum as a label for fluorescence immunohistochemistry. Both Fluoro-Gold and AMCA are excited by near-ultraviolet (UV) light, but under UV excitation Fluoro-Gold appears yellow and AMCA appears blue. Thus the two fluorochromes are both visible under a single condition of illumination and can be readily distinguished by color. This allows quick and accurate determination of whether or not immunofluorescent fibers appose retrogradely labeled neurons.

References

Mar 15, 1979·The Journal of Comparative Neurology·R P BarberS E Leeman
Sep 1, 1986·The Histochemical Journal·H KhalfanD Robinson
Oct 14, 1967·Nature·M StefaniniL Zamboni
Jan 1, 1981·Journal of Immunological Methods·G D Johnson, G M Nogueira Araujo
Feb 10, 1981·The Journal of Comparative Neurology·T A RandoR E Zigmond
Feb 9, 1965·Zeitschrift für Zellforschung und mikroskopische Anatomie·K FUXE

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Citations

Jul 1, 1989·Journal of Neuroscience Methods·G JuL Z Fan
Nov 1, 2003·Biochemical and Biophysical Research Communications·K CzajaC R Barb
Feb 8, 2003·Experimental Eye Research·Victor M ElnerHoward R Petty
Oct 1, 1991·The Journal of Comparative Neurology·C T LundquistD R Nässel
Jul 1, 2010·The Journal of Comparative Neurology·Wenyu FuTomas Hökfelt

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