PMID: 7011946Jan 1, 1981Paper

Simultaneous occurrence of pregnancylike lobuloalveolar morphogenesis and casein-gene expression in a culture of the whole mammary gland

In Vitro
N GangulyM R Banerjee

Abstract

Entire second thoracic mammary glands of estrogen- and progesterone-treated immature virgin BALB/c mice were stimulated to pregnancylike lobuloalveolar morphogenesis after 6 days of incubation with insulin (5 micrograms/ml), aldosterone (1 micrograms/ml), growth hormone (5 micrograms/ml), cortisol (5 micrograms/ml), and prolactin (80 ng/ml, present as a contaminant in 5 micrograms/ml growth hormone). The alveolar growth in the glands, as judged by morphological studies, was accompanied by an increase in cell number as a function of incubation time in the hormonal medium. Hybridization of the total RNA from these glands to the casein mRNA specific complementary DNA probe (cDNAcsn) revealed that the level of casein mRNA rises from 0.00012 to 0.005% between 1 and 6 days of incubation. Estimates showed that the concentration of casein mRNA per cell rises 17-fold from 70 molecules on Day 1 to 1200 molecules on Day 6, whereas the number of epithelial cells increases only twofold during the same incubation time. When the growth hormone preparation was totally replaced by 80 ng of prolactin during the 6-day incubation, casein-mRNA levels were found to be 0.0083%. These results demonstrate that a pregnancy-like morphogenesis and concurr...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N T TelangA B Kundu
Jan 1, 1976·International Review of Cytology·M R Banerjee
Jan 4, 1979·Biochimica Et Biophysica Acta·S Sakai, M R Banerjee
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R GangulyM R Banerjee
Dec 1, 1977·Molecular and Cellular Endocrinology·P M TerryM R Banerjee
Jan 1, 1978·In Vitro·M R BanerjeeR Ganguly
Aug 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·W G JuergensJ J Elias
Mar 1, 1970·Endocrinology·Y AmenomoriJ Meites
May 1, 1969·The Journal of Endocrinology·N J Kuhn
Aug 1, 1966·The Journal of Endocrinology·R R Ichinose, S Nandi
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R GangulyM R Banerjee
Sep 1, 1961·General and Comparative Endocrinology·S NANDI, H A BERN

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Citations

May 12, 2000·Proceedings of the Society for Experimental Biology and Medicine·T M CaseyK Plaut
Jun 15, 1987·International Journal of Cancer. Journal International Du Cancer·S ChakrabortyM R Banerjee
Dec 18, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·E Gallo-HendrikxZ X Xiao

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