Simultaneous photoacoustic microscopy of microvascular anatomy, oxygen saturation, and blood flow

Optics Letters
Bo NingSong Hu

Abstract

Capitalizing on the optical absorption of hemoglobin, photoacoustic microscopy (PAM) is uniquely capable of anatomical and functional characterization of the intact microcirculation in vivo. However, PAM of the metabolic rate of oxygen (MRO<sub>2</sub>) at the microscopic level remains an unmet challenge, mainly due to the inability to simultaneously quantify microvascular diameter, oxygen saturation of hemoglobin (sO<sub>2</sub>), and blood flow at the same spatial scale. To fill this technical gap, we have developed a multi-parametric PAM platform. By analyzing both the sO<sub>2</sub>-encoded spectral dependence and the flow-induced temporal decorrelation of photoacoustic signals generated by the raster-scanned mouse ear vasculature, we demonstrated-for the first time-simultaneous wide-field PAM of all three parameters down to the capillary level in vivo.

References

May 3, 2008·Optics Letters·Konstantin MaslovLihong V Wang
Aug 3, 2011·Journal of Biomedical Optics·Junjie YaoLihong V Wang
Jan 13, 2012·Cell Death & Disease·C Muñoz-PinedoJ-E Ricci
Apr 8, 2014·Current Radiology Reports·Hongyu AnWeili Lin

❮ Previous
Next ❯

Citations

Dec 8, 2015·Photoacoustics·P J van den BergW Steenbergen
Apr 26, 2016·Current Opinion in Chemical Biology·Song Hu
Oct 21, 2016·Photoacoustics·Wenzhong Liu, Hao F Zhang
Jan 26, 2017·Plastic and Reconstructive Surgery·Brent R DeGeorgeChristopher A Campbell
Apr 28, 2018·Journal of Biomedical Optics·Christopher MirandaBarbara Smith
Mar 1, 2018·Journal of Biomedical Optics·Thore M BückingJoanna Brunker
Jun 17, 2018·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Penn Mason McClatcheyDavid H Wasserman
Nov 29, 2016·Journal of Biomedical Optics·Paul Kumar Upputuri, Manojit Pramanik
Feb 10, 2016·Journal of Biomedical Optics·Pim J van den BergWiendelt Steenbergen
Feb 18, 2016·Journal of Biomedical Optics·Chenghung YehLihong V Wang
Nov 2, 2016·Hypertension·T C Stevenson KellerBrant E Isakson
Dec 6, 2018·Journal of Biophotonics·Zhendong GuoSung-Liang Chen
Oct 30, 2019·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Naidi SunSong Hu
Feb 13, 2020·Journal of Biophotonics·Yingying ZhouLidai Wang
Apr 8, 2017·Journal of Optics·Yong Ku ChoYoko Hoshi
Feb 11, 2020·Annals of Plastic Surgery·Patrick S CottlerChris A Campbell
Apr 19, 2017·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Wanrong Gao
Dec 28, 2018·Light, Science & Applications·Stephan KellnbergerVasilis Ntziachristos
Feb 3, 2016·Neurophotonics·Utku Baran, Ruikang K Wang
Sep 17, 2017·Nature Communications·Michael F GutknechtKimberly A Kelly
Feb 9, 2019·Scientific Reports·Vaskar GnyawaliMichael C Kolios
Jan 16, 2019·Optics Letters·Tianxiong WangSong Hu
Nov 5, 2019·Journal of Imaging·Van Phuc Nguyen, Yannis M Paulus
Nov 22, 2018·Biomedical Optics Express·Sixian YouHaohua Tu
Dec 20, 2019·Tissue Engineering. Part B, Reviews·Binita ShresthaEric M Brey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.