Simultaneous polychromatic flow cytometric detection of multiple forms of regulated cell death

Apoptosis : an International Journal on Programmed Cell Death
D BergamaschiG Warnes

Abstract

Currently the study of Regulated Cell Death (RCD) processes is limited to the use of lysed cell populations for Western blot analysis of each separate RCD process. We have previously shown that intracellular antigen flow cytometric analysis of RIP3, Caspase-3 and cell viability dye allowed the determination of levels of apoptosis (Caspase-3+ ve/RIP3- ve), necroptosis (RIP3Hi + ve/Caspase-3- ve) and RIP1-dependent apoptosis (Caspase-3+ ve/RIP3+ ve) in a single Jurkat cell population. The addition of more intracellular markers allows the determination of the incidence of parthanatos (PARP), DNA Damage Response (DDR, H2AX), H2AX hyper-activation of PARP (H2AX/PARP) autophagy (LC3B) and ER stress (PERK), thus allowing the identification of 124 sub-populations both within live and dead cell populations. Shikonin simultaneously induced Jurkat cell apoptosis and necroptosis the degree of which can be shown flow cytometrically together with the effects of blockade of these forms of cell death by zVAD and necrostatin-1 have on specific RCD populations including necroptosis, early and late apoptosis and RIP1-dependent apoptosis phenotypes in live and dead cells. Necrostatin-1 and zVAD was shown to modulate levels of shikonin induced DDR,...Continue Reading

References

Apr 13, 2004·Oncogene·Maria CastedoGuido Kroemer
May 22, 2007·Molecular Cancer Therapeutics·Weidong HanXun Hu
Jan 5, 2010·Current Opinion in Cell Biology·Andrew Rj Young, Masashi Narita
Apr 1, 2010·Science Signaling·Peter VandenabeeleTom Vanden Berghe
Mar 7, 2012·Genes & Development·Xin Luo, W Lee Kraus
Nov 3, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Benjamin WienchThomas Efferth
Jul 13, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Shaheen ChikteGary Warnes
Jan 21, 2014·Cell·Wan Seok YangBrent R Stockwell
Mar 4, 2014·Critical Reviews in Eukaryotic Gene Expression·Julio MoralesDavid A Boothman
Mar 29, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Zbigniew DarzynkiewiczJoseph M Wu
Apr 2, 2014·British Journal of Pharmacology·Amos A FatokunTed M Dawson
Sep 23, 2014·Cell Death and Differentiation·L GalluzziG Kroemer
Jan 28, 2015·International Journal of Molecular Sciences·Piotr CzarnyJanusz Blasiak
Feb 7, 2015·Trends in Biochemical Sciences·Daniela Senft, Ze'ev A Ronai
Apr 8, 2015·Methods : a Companion to Methods in Enzymology·G Warnes
May 6, 2015·Oncoimmunology·Oliver KeppLorenzo Galluzzi
Dec 3, 2015·Cold Spring Harbor Perspectives in Biology·Douglas R Green, Fabien Llambi
Jan 19, 2016·Nature Reviews. Drug Discovery·Marcus ConradBrent R Stockwell
Oct 16, 2016·International Journal of Molecular Sciences·Hirofumi Sawai
Nov 28, 2017·Methods : a Companion to Methods in Enzymology·H L LeeG Warnes
Jan 25, 2018·Cell Death and Differentiation·Lorenzo GalluzziGuido Kroemer
Nov 20, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·A PopatG Warnes

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Citations

Mar 9, 2020·Apoptosis : an International Journal on Programmed Cell Death·Patrycja Nowak-Sliwinska, Arjan W Griffioen
Sep 7, 2019·International Journal of Molecular Sciences·Anna Vossenkamper, Gary Warnes
Jun 5, 2020·Apoptosis : an International Journal on Programmed Cell Death·G Warnes
Nov 29, 2020·Cellular and Molecular Gastroenterology and Hepatology·Wing-Yiu Jason LeeGraham R Foster
Mar 23, 2021·Frontiers in Cell and Developmental Biology·Xi-Min HuKun Xiong
Jan 14, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Heedoo LeeYang Jin
May 28, 2021·Veterinary Pathology·Sara Francesca SantagostinoEnrico Radaelli

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Methods Mentioned

BETA
flow cytometry
Fluorescence

Software Mentioned

GraphPad

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