Simultaneous quantification of capsaicin and dihydrocapsaicin in rat plasma using HPLC coupled with tandem mass spectrometry

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Qinghao ZhangYan Li

Abstract

A rapid, simple and sensitive HPLC-ESI-MS/MS method was developed for the simultaneous determination of capsaicin and dihydrocapsaicin in rat plasma. Plasma samples containing capsaicin, dihydrocapsaicin and phenacetin (internal standard) were prepared based on a simple protein precipitation by the addition of two volumes of acetonitrile. The analytes and internal standard were separated on a Zorbax SB-C18 column (3.5microm, 2.1mmx100mm) with mobile phase of acetonitrile/water (55:45, v/v) containing 0.1% formic acid (v/v) at a flow rate of 0.2mL/min with an operating temperature of 25 degrees C. Quantification was performed on a triple quadrupole mass spectrometer equipped with electrospray ionization (ESI) source by selected reaction monitoring (SRM) of the transitions at m/z 306-137 for capsaicin, m/z 308-137 for dihydrocapsaicin and m/z 180-110 for the IS. Linear detection responses were obtained for capsaicin and dihydrocapsaicin ranging from 1 to 500ng/mL and the lower limits of quantitation (LLOQs) for the two compounds were 1ng/mL. The intra- and inter-day precisions (R.S.D.%) were within 9.79% for the two analytes, while the deviations of assay accuracies were within +/-10.63%. The average recoveries of the analytes we...Continue Reading

Citations

Dec 5, 2012·Chemistry Central Journal·Monica ButnariuSalomeia Putnoky
Dec 3, 2014·Journal of Pharmaceutical and Biomedical Analysis·Mónika KuzmaPál Perjési
Nov 5, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alberto González-ZamoraJosé L García-Hernández
Jan 1, 2014·Phytochemical Analysis : PCA·Ju-Hee Oh, Young-Joo Lee
Oct 20, 2012·Journal of Chromatographic Science·Haejin BaeBhimanagouda S Patil
Sep 7, 2016·Anais Da Academia Brasileira De Ciências·Lorena M D CirinoJuliana C Cardoso
Feb 1, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria de Lourdes Reyes-EscogidoErika Vazquez-Tzompantzi
Aug 8, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mónika KuzmaPál Perjési
Dec 24, 2018·Foods·Carolina Cortés-HerreraFabio Granados-Chinchilla
Jul 12, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yan WangJian-Shan Ye

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