PMID: 11318407Apr 25, 2001Paper

Simultaneous quantification of components of neoglycolipid-coated liposomes using high-performance liquid chromatography with evaporative light scattering detection

Journal of Chromatography. B, Biomedical Sciences and Applications
Y ShimizuT Mizuochi

Abstract

Liposomes composed of dipalmitoylphosphatidylcholine (DPPC), cholesterol and a neoglycolipid, mannopentaose-conjugated dipalmitoylphosphatidylethanolamine (Man5-DPPE), have been shown to have a strong adjuvant effect in inducing the antigen-specific cellular immunity. In this study, a rapid and simple analytical method using a HPLC system with an evaporative light scattering detector was developed for simultaneous quantification of the liposome components Man5-DPPE, cholesterol and DPPC. The chromatographic separation of these components was performed using a trimethylsilane column with an isocratic mobile phase of chloroform-methanol-water (1:33:6, v/v) after disrupting the liposomes with chloroform-methanol-water (10:10:3, v/v). This HPLC method provided sufficient reproducibility and linearity of calibration curves for the quantification of the liposome constituents. In addition, this method can be used for the quantification of various neoglycolipids with different carbohydrate structures.

References

Aug 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·M TakayamaI Tanimizu
Feb 1, 1996·European Journal of Immunology·A AvraméasP Midoux
Jul 24, 1998·Trends in Biotechnology·D D Lasic

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Citations

Nov 10, 2009·Journal of Pharmaceutical and Biomedical Analysis·Zhenming ZhongJ Allen Zhang
Mar 12, 2003·Bioorganic & Medicinal Chemistry·Yoshitaka ShimizuNaoya Kojima
May 30, 2006·The Journal of Parasitology·Noritaka KubokiChihiro Sugimoto
Aug 18, 2020·Critical Reviews in Analytical Chemistry·Guangyan LiuJianping Li
Oct 5, 2020·Journal of Pharmaceutical and Biomedical Analysis·Yuchen FanKelly Zhang

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