Simultaneous quantification of fentanyl, sufentanil, cefazolin, doxapram and keto-doxapram in plasma using liquid chromatography-tandem mass spectrometry

Biomedical Chromatography : BMC
Robert B FlintBirgit C P Koch

Abstract

A simple and specific UPLC-MS/MS method was developed and validated for simultaneous quantification of fentanyl, sufentanil, cefazolin, doxapram and its active metabolite keto-doxapram. The internal standard was fentanyl-d5 for all analytes. Chromatographic separation was achieved with a reversed-phase Acquity UPLC HSS T3 column with a run-time of only 5.0 min per injected sample. Gradient elution was performed with a mobile phase consisting of ammonium acetate or formic acid in Milli-Q ultrapure water or in methanol with a total flow rate of 0.4 mL min-1 . A plasma volume of only 50 μL was required to achieve adequate accuracy and precision. Calibration curves of all five analytes were linear. All analytes were stable for at least 48 h in the autosampler. The method was validated according to US Food and Drug Administration guidelines. This method allows quantification of fentanyl, sufentanil, cefazolin, doxapram and keto-doxapram, which is useful for research as well as therapeutic drug monitoring, if applicable. The strength of this method is the combination of a small sample volume, a short run-time, a deuterated internal standard, an easy sample preparation method and the ability to simultaneously quantify all analytes in ...Continue Reading

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Citations

Sep 10, 2019·Archives of Disease in Childhood. Fetal and Neonatal Edition·Swantje VöllerUNKNOWN DINO study group
Jul 23, 2020·Pediatric Research·Robert B FlintUNKNOWN DINO Research Group
Nov 2, 2020·Journal of Pharmaceutical and Biomedical Analysis·Nutnicha Chansud, Opas Bunkoed

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Methods Mentioned

BETA
sedation
blood donation

Clinical Trials Mentioned

NCT02421068

Software Mentioned

Xcalibur
Chromeleon
LCquan

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