Simultaneous quantification of three active alkaloids from a traditional Chinese medicine Ramulus Mori (Sangzhi) in rat plasma using liquid chromatography-tandem mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis
Shuang YangYan Li

Abstract

Fagomine, 1-deoxynojirimycin (DNJ) and 1,4-dideoxy-1,4-imino-d-arabinitol (DAB) are the major bioactive constituents in the active fraction of alkaloids from the traditional Chinese medicine mulberry twig (Ramulus Mori, Chinese name Sang Zhi), which has a strong activity on α-glucosidase in vitro and in vivo. A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of DNJ, fagomine and DAB in rat plasma. Plasma samples were prepared using a simple protein precipitation by the addition of 1% volume of Tris and two volumes of methanol-acetonitrile. The analytes and internal standard (IS, miglitol) were chromatographed in an XBridge™ amide column with a gradient mobile phase of acetonitrile-water (0.1% ammonium hydroxide) at a flow rate of 0.7mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization (ESI) source in positive ion mode by multiple reaction monitoring (MRM) mode. Linear detection responses were obtained for DNJ ranging from 5.00 to 5000.00ng/mL, 10.00 to 2500.00ng/mL for fagomine and DAB. The lower limits of quantification (LLOQs) were 5.00, 10.00, 10.00ng/mL for ...Continue Reading

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Citations

Mar 8, 2016·Talanta·Susana Amézqueta, Josep Lluís Torres
Nov 3, 2016·Journal of Agricultural and Food Chemistry·Yun-Chong ZhengYan-Hua Lu
May 11, 2017·Journal of Agricultural and Food Chemistry·Susana AmézquetaJosep Lluís Torres
May 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhihua LiuYuling Liu
Jan 10, 2020·Critical Reviews in Analytical Chemistry·Kun-Qiang HongZhi-Wen Wang
Mar 3, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wansha YuNingjia He
Jan 19, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Mengran XuXin Sun

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