Simultaneous Quantification of Viral Antigen Expression Kinetics Using Data-Independent (DIA) Mass Spectrometry.

Molecular & Cellular Proteomics : MCP
Nathan P CroftAnthony W Purcell

Abstract

The generation of antigen-specific reagents is a significant bottleneck in the study of complex pathogens that express many hundreds to thousands of different proteins or to emerging or new strains of viruses that display potential pandemic qualities and therefore require rapid investigation. In these instances the development of antibodies for example can be prohibitively expensive to cover the full pathogen proteome, or the lead time may be unacceptably long in urgent cases where new highly pathogenic viral strains may emerge. Because genomic information on such pathogens can be rapidly acquired this opens up avenues using mass spectrometric approaches to study pathogen antigen expression, host responses and for screening the utility of therapeutics. In particular, data-independent acquisition (DIA) modalities on high-resolution mass spectrometers generate spectral information on all components of a complex sample providing depth of coverage hitherto only seen in genomic deep sequencing. The spectral information generated by DIA can be iteratively interrogated for potentially any protein of interest providing both evidence of protein expression and quantitation. Here we apply a solely DIA mass spectrometry based methodology t...Continue Reading

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Citations

Jul 6, 2015·Current Opinion in Microbiology·Saskia Kreibich, Wolf-Dietrich Hardt
Apr 10, 2016·Current Opinion in Immunology·Anthony W PurcellDavid C Tscharke
Aug 4, 2018·Molecular & Cellular Proteomics : MCP·Bracha ShraibmanArie Admon
Jun 4, 2019·Molecular & Cellular Proteomics : MCP·Bracha ShraibmanArie Admon
Jul 9, 2020·International Journal of Molecular Sciences·Susannah HallalKimberley Louise Alexander-Kaufman
Aug 8, 2020·Journal of Virology·Sarah CroftDavid C Tscharke
Feb 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Nathan P CroftDavid C Tscharke
Nov 1, 2018·Molecular & Cellular Proteomics : MCP·Bracha ShraibmanArie Admon
Jun 1, 2019·Molecular & Cellular Proteomics : MCP·Bracha ShraibmanArie Admon

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