Simultaneous quantitation of seven endogenous C-21 adrenal steroids by liquid chromatography tandem mass spectrometry in human serum

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Valdemir M CarvalhoJosé G H Vieira

Abstract

Quantitation of endogenous steroids is important in the diagnosis of several endocrine disorders. In this study we present a new method for simultaneous quantitation of cortisol, cortisone, 11-desoxycortisol, 21-desoxycortisol, corticosterone, 17-hydroxyprogesterone and 11-desoxycorticosterone in human serum by on-line extraction and LC-MS/MS. Analytes extraction was performed on-line using a 2-position and 6-port valve equipped with a monolithic silica cartridge. After chromatographic separation of all analytes, detection was performed in the multiple reaction monitoring mode using positive atmospheric pressure chemical ionization mode. Total imprecision of the assay ranged from 5.5 to 15.5%. Comparison with immunoassays yielded coefficients of 0.893 for cortisol, 0.848 for 11-desoxycortisol and 0.924 for 17-hydroxyprogesterone. The sensitivity of this method provides meaningful data for patients within normal and elevated range and it may be useful for the diagnosis of a variety of adrenal dysfunctions.

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Citations

Apr 16, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wei GaoClemens Kirschbaum
Apr 23, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhixiang YanXinghao Yang
Feb 16, 2011·Bioanalysis·Dong Sheng MingTeri-Lynn Bajkov
Oct 15, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Mirko PeitzschGraeme Eisenhofer
Dec 18, 2013·Bioanalysis·Mariëtte Theodora Ackermans, Erik Endert
Jun 21, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Pekka Keski-RahkonenSeppo Auriola
Jul 16, 2010·Clinical Biochemistry·Mark M KushnirA Wayne Meikle
Nov 26, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Claudia RossiMichael Morris
Apr 4, 2016·Analytical and Bioanalytical Chemistry·Ashley S P BoggsJohn R Kucklick
Jul 17, 2010·Rapid Communications in Mass Spectrometry : RCM·M GalesioJ L Capelo-Martínez
May 7, 2016·Analytical and Bioanalytical Chemistry·Johan Juhl WeisserBjarne Styrishave
Feb 19, 2020·Clinical Chemistry and Laboratory Medicine : CCLM·Caroline Le GoffEtienne Cavalier
May 28, 2014·Arquivos brasileiros de endocrinologia e metabologia·João Luiz de Oliveira MadeiraBerenice Bilharinho de Mendonça
Dec 4, 2014·Arquivos brasileiros de endocrinologia e metabologia·José Gilberto Henriques VieiraValdemir Melechko Carvalho
May 1, 2015·The Journal of Pharmacology and Experimental Therapeutics·Yi ZhuQing-Yu Zhang
Sep 13, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Valdemir Melechco Carvalho

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