Simultaneous quantitation of two diastereoisomers of lobaplatin in rat plasma by supercritical fluid chromatography with tandem mass spectrometry and its application to a pharmacokinetic study

Journal of Separation Science
Xiangjun MengJingkai Gu

Abstract

Lobaplatin, consisting of two diastereoisomers, is a third-generation platinum antineoplastic agent that has shown encouraging anticancer activity in a variety of tumor types. To investigate any stereospecificity in the pharmacokinetics of lobaplatin, a novel, simple, rapid and sensitive supercritical fluid chromatography with tandem mass spectrometry method was developed for the simultaneous quantitation of lobaplatin diastereoisomers in rat plasma. After a simple protein precipitation with methanol, the analytes and dexpantoprazole (internal standard) were chromatographed on an Acquity UPC(2) system with a Chiralcel OZ-RH column using a mobile phase consisting of carbon dioxide and methanol (65:35, v/v) at 40°C over 6 min. The assay was linear over a concentration range of 25-15,000 ng/mL for both diastereoisomers using 100 μL of rat plasma for sample preparation. The lower limit of quantification was 25 ng/mL for both compounds, which was sufficient to detect the diastereoisomers in the incurred samples within this study. Intra- and inter-day precisions were below 11.8% and the accuracies were below 4.5%. The validated method was successfully applied to a pharmacokinetic study after an intravenous administration of 7.6 mg/kg...Continue Reading

References

Nov 1, 1990·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·L H Einhorn
Sep 1, 1993·Drugs·Raymond B Weiss, Michaele C Christian
Jun 1, 1995·British Journal of Cancer·J A GietemaE G de Vries
Apr 1, 1996·Diabetes Care·G G Dahlquist, B A Källen
Jul 24, 1997·The New England Journal of Medicine·G J Bosl, R J Motzer
Mar 30, 2000·Analytical Chemistry·K W Phinney
Dec 16, 2000·Expert Opinion on Investigational Drugs·M J McKeage
Oct 31, 2003·Drugs in R&D
Apr 20, 2005·Drug Discovery Today·Yingru ZhangAdrienne A Tymiak
Mar 1, 2012·Journal of Pharmaceutical and Biomedical Analysis·Katrijn De KlerckYvan Vander Heyden
May 16, 2012·Anti-cancer Agents in Medicinal Chemistry·Imran AliAshanul Haque
Jun 13, 2012·Journal of Pharmaceutical and Biomedical Analysis·A J AlexanderF P Tomasella
Jul 10, 2013·Journal of Separation Science·Min LiQilong Ren
Dec 18, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yajie GengTianhong Zhang
Apr 8, 2014·Analytica Chimica Acta·Květa KalíkováEva Tesařová
Dec 19, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Xu SunXiao-Hua Wu
Feb 3, 2015·Journal of Separation Science·Norma S Pano-FariasRoberto Muñiz-Valencia
Feb 11, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fangfang YangYuquan Wei

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