Simultaneous quantitative determination of paracetamol and tramadol in tablet formulation using UV spectrophotometry and chemometric methods

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Siniša GlavanovićVladislav Tomišić

Abstract

The UV spectrophotometric methods for simultaneous quantitative determination of paracetamol and tramadol in paracetamol-tramadol tablets were developed. The spectrophotometric data obtained were processed by means of partial least squares (PLS) and genetic algorithm coupled with PLS (GA-PLS) methods in order to determine the content of active substances in the tablets. The results gained by chemometric processing of the spectroscopic data were statistically compared with those obtained by means of validated ultra-high performance liquid chromatographic (UHPLC) method. The accuracy and precision of data obtained by the developed chemometric models were verified by analysing the synthetic mixture of drugs, and by calculating recovery as well as relative standard error (RSE). A statistically good agreement was found between the amounts of paracetamol determined using PLS and GA-PLS algorithms, and that obtained by UHPLC analysis, whereas for tramadol GA-PLS results were proven to be more reliable compared to those of PLS. The simplest and the most accurate and precise models were constructed by using the PLS method for paracetamol (mean recovery 99.5%, RSE 0.89%) and the GA-PLS method for tramadol (mean recovery 99.4%, RSE 1.69%).

References

Aug 6, 2003·Journal of Pharmaceutical and Biomedical Analysis·E M P J GarridoC Delerue-Matos
Apr 15, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hans Jörg LeisWerner Windischhofer
Oct 29, 2004·Clinical Pharmacokinetics·Stefan Grond, Armin Sablotzki
Sep 11, 2007·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·M R KhoshayandA Mohammadi
Sep 25, 2008·Paediatric Anaesthesia·Brian J Anderson
Dec 9, 2008·Journal of Pharmaceutical and Biomedical Analysis·Bhavin N PatelPranav S Shrivastav
Jul 9, 2010·Journal of Pharmaceutical and Biomedical Analysis·E ZiémonsPh Hubert
Dec 25, 2012·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Julio Cesar L Alves, Ronei J Poppi
May 6, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mahmoud M ElkhoudaryGhada M Hadad
Jun 24, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Shereen M TawakkolMostafa A Shehata

❮ Previous
Next ❯

Citations

Jan 2, 2021·Forensic Science International : Synergy·Nicole S Jones, Jeffrey H Comparin
Feb 25, 2021·Critical Reviews in Analytical Chemistry·R RamaS C B Gopinath
May 14, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Ahmed ElsonbatyMaya S Eissa
Jun 13, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mortaza IranifamHaider A J Al Lawati
Apr 13, 2018·ACS Applied Materials & Interfaces·Ayomi S PereraMohan Edirisinghe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.