Simultaneous recording of calcium signals from identified neurons and feeding behavior of Drosophila melanogaster.

Journal of Visualized Experiments : JoVE
Motojiro Yoshihara

Abstract

To study neuronal networks in terms of their function in behavior, we must analyze how neurons operate when each behavioral pattern is generated. Thus, simultaneous recordings of neuronal activity and behavior are essential to correlate brain activity to behavior. For such behavioral analyses, the fruit fly, Drosophila melanogaster, allows us to incorporate genetically encoded calcium indicators such as GCaMP(1), to monitor neuronal activity, and to use sophisticated genetic manipulations for optogenetic or thermogenetic techniques to specifically activate identified neurons(2-5). Use of a thermogenetic technique has led us to find critical neurons for feeding behavior (Flood et al., under revision). As a main part of feeding behavior, a Drosophila adult extends its proboscis for feeding(6) (proboscis extension response; PER), responding to a sweet stimulus from sensory cells on its proboscis or tarsi. Combining the protocol for PER(7) with a calcium imaging technique(8) using GCaMP3.0(1, 9), I have established an experimental system, where we can monitor activity of neurons in the feeding center - the suboesophageal ganglion (SOG), simultaneously with behavioral observation of the proboscis. I have designed an apparatus (...Continue Reading

References

Jun 13, 2008·Nature·Fumika N HamadaPaul A Garrity
Nov 4, 2008·Journal of Visualized Experiments : JoVE·Takashi Shiraiwa, John R Carlson
Feb 17, 2009·Neuron·Michael D Gordon, Kristin Scott
Mar 20, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nathan C PeabodyBenjamin H White

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Citations

Oct 30, 2012·Nature·Christopher J BurkeScott Waddell
Jun 12, 2013·Nature·Thomas F FloodMotojiro Yoshihara
Sep 30, 2014·Nature Neuroscience·Suewei LinScott Waddell
Aug 28, 2019·PLoS Biology·Dominique SiegenthalerJan Pielage
May 6, 2016·PLoS Biology·Ashiq HussainIlona C Grunwald Kadow
May 6, 2016·PLoS Biology·Ashiq HussainIlona C Grunwald Kadow
Mar 8, 2019·Nature Communications·Mathias A BöhmeAlexander M Walter
Mar 14, 2019·Frontiers in Cellular Neuroscience·Madhuvanthi KannanVincent A Pieribone

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