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Simultaneous repression of multiple bacterial genes using nonrepetitive extra-long sgRNA arrays

Nature Biotechnology

Oct 9, 2019

Alexander C ReisHoward M Salis

Abstract

Engineering cellular phenotypes often requires the regulation of many genes. When using CRISPR interference, coexpressing many single-guide RNAs (sgRNAs) triggers genetic instability and phenotype loss, due to the presence of repetitive DNA sequences. We stably coexpressed 22 sgRNAs wit...read more

Mentioned in this Paper

Biological Adaptation to Stress
Genetically Engineered Mouse
Gene Knockdown Techniques
Genes
CRISPR-Cas Systems
Regulation of Biological Process
Genetic Activator
Binding (Molecular Function)
DNA Replication
Genomic Instability
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Simultaneous repression of multiple bacterial genes using nonrepetitive extra-long sgRNA arrays

Nature Biotechnology

Oct 9, 2019

Alexander C ReisHoward M Salis

PMID: 31591552

DOI: 10.1038/s41587-019-0286-9

Abstract

Engineering cellular phenotypes often requires the regulation of many genes. When using CRISPR interference, coexpressing many single-guide RNAs (sgRNAs) triggers genetic instability and phenotype loss, due to the presence of repetitive DNA sequences. We stably coexpressed 22 sgRNAs wit...read more

Mentioned in this Paper

Biological Adaptation to Stress
Genetically Engineered Mouse
Gene Knockdown Techniques
Genes
CRISPR-Cas Systems
Regulation of Biological Process
Genetic Activator
Binding (Molecular Function)
DNA Replication
Genomic Instability
324
1
109
1
1

Similar Papers Found In These Feeds

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

DNA Damage

Cells are subjected to several DNA damaging events on a daily basis which results in the induction of DNA damage signaling and repair cascades to ensure genomic stability. Here is the latest research pertaining to DNA damage.

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Orthologous CRISPR-Cas9 enzymes for combinatorial genetic screens

Nature BiotechnologyDecember 19, 2017
Fadi J NajmJohn G Doench
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